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首页> 外文期刊>Biomaterials >Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress.
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Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress.

机译：在剪切应力的作用下，人内皮细胞对血管修复材料的附着，形态和粘附。

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In an effort to improve the long-term patency of vascular prostheses several groups now advocate seeding autologous endothelial cells (ECs) onto the lumen of the vessel prior to implantation, a procedure that involves pre-treating the prosthesis material with fibrin, collagen and/or other matrix molecules to promote cell attachment and retention. In this study, we examined the degree to which human umbilical venous endothelial cells (HUVECs) adhered to three materials commonly used polymeric vascular prosthesis that had been coated with the same commercial extra cellular matrix proteins, and after exposure to fluid shear stresses representative of femoro-distal bypass in a cone-and-plate shearing device. We quantified cell number, area of coverage and degree of cell spreading using image analysis techniques. The response of cells that adhered to the surface of each material, and following exposure to fluid shear stress, depended on surface treatment, topology and cell type. Whereas collagen coating improved primary cellular adhesion and coverage significantly, the degree of spreading depended on the underlying surface structure and on the application of the shear stress. In some cases, fewer than 30% of cells remained on the surface after only 1-h exposure to physiological levels of shear stress. The proportion of the surface that was covered by cells also decreased, despite an increase in the degree to which individual cells spread on exposure to shear stress. Moreover, the behaviour of HUVECs was distinct from that of fibroblasts, in that the human ECs were able to adapt to their environment by spreading to a much greater extent in response to shear. The quality of HUVEC attachment, as measured by extent of cell coverage and resistance to fluid shear stress, was greatest on expanded polytetrafluoroethylene samples that had been impregnated with Type I/III collagen.
机译：为了改善血管假体的长期通畅性，现在有几个小组主张在植入前在血管腔内植入自体内皮细胞（EC），该过程涉及用纤维蛋白，胶原蛋白和/或对假体材料进行预处理。或其他基质分子以促进细胞附着和保留。在这项研究中，我们研究了人脐静脉内皮细胞（HUVEC）粘附到三种材料的普遍程度，这些材料已经涂有相同的商业细胞外基质蛋白，并且在暴露于代表股骨的液体切应力后锥板剪切装置中的远侧旁路。我们使用图像分析技术量化细胞数量，覆盖面积和细胞扩散程度。粘附到每种材料表面的细胞的响应以及暴露于流体剪切应力后的响应取决于表面处理，拓扑和细胞类型。胶原蛋白涂层可显着改善原代细胞的粘附力和覆盖率，而铺展的程度则取决于下面的表面结构和剪切应力的施加。在某些情况下，仅在暴露于生理水平的切应力1小时后，不到30％的细胞保留在表面上。尽管单个细胞暴露于剪切应力下的扩散程度有所增加，但被细胞覆盖的表面比例也有所降低。此外，HUVEC的行为与成纤维细胞的行为不同，因为人类EC能够通过响应剪切力而更大程度地扩散而适应环境。通过细胞覆盖程度和对流体剪切应力的抵抗力来衡量，HUVEC附着的质量在已浸有I / III型胶原的膨胀聚四氟乙烯样品上最高。

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