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EGF-grafted PDMS surfaces in artificial cornea applications

机译：EGF接枝的PDMS表面在人造角膜中的应用

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Lack of epithelial cell coverage has remained a persistent problem in the design of an artificial cornea. In this work, polydimethylsiloxane (PDMS) surfaces were modified with epidermal growth factor (EGF) to improve the growth of corneal epithelial cells. The EGF was covalently tethered to PDMS substrates aminated by plasma polymerization of allylamine via a homobifunctional polyethylene glycol (PEG) spacer. Surface modification was confirmed by contact angle and X-ray photoelectron spectroscopy measurements. By varying the ratio of EGF to PEG from 1:50 to 1:5, EGF amounts from 40 to 90ng/cm(2) could be bound, as determined by surface plasmon resonance (SPR) and I-125 radiolabelling. Human corneal epithelial cells on the various modified surfaces were cultured both in the presence and absence of EGF in the culture medium to determine the effect of covalently bound EGF on the cells. The results demonstrated that covalently bound EGF on the surfaces is active with respect to promoting epithelial cell coverage. This was significant when compared to unmodified controls. (c) 2005 Elsevier Ltd. All rights reserved.
机译：缺乏上皮细胞覆盖一直是人工角膜设计中的持续问题。在这项工作中，用表皮生长因子（EGF）修饰了聚二甲基硅氧烷（PDMS）表面，以改善角膜上皮细胞的生长。 EGF通过同双功能聚乙二醇（PEG）间隔基与烯丙胺的等离子聚合胺化的PDMS底物共价连接。通过接触角和X射线光电子能谱测量来确认表面改性。通过将EGF与PEG的比例从1:50更改为1：5，可以结合EGF的量为40至90ng / cm（2），这由表面等离振子共振（SPR）和I-125放射性标记确定。在培养基中存在和不存在EGF的情况下，在各种修饰的表面上培养人角膜上皮细胞，以确定共价结合的EGF对细胞的作用。结果证明，在表面上共价结合的EGF对于促进上皮细胞覆盖具有活性。与未修改的对照相比，这很重要。 （c）2005 Elsevier Ltd.保留所有权利。

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