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首页> 外文期刊>Biomaterials >Production and characterization of ECM powder: implications for tissue engineering applications.
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Production and characterization of ECM powder: implications for tissue engineering applications.

机译：ECM粉末的生产和表征：对组织工程应用的意义。

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Two methods to produce a particulate form of extracellular matrix (ECM) from porcine urinary bladder were investigated. One method to produce the powder involved snap freezing a lyophilized form of the material and then pulverizing it in a grinding mill. The second method was similar except that the ECM was saturated in a solution of NaCl prior to snap freezing to precipitate salt crystals within the matrix before grinding. Several methods were utilized to analyze the particle size distribution and ultrastructure including sonic sifting, laser diffraction, and scanning electron microscopy (SEM). The salt precipitation method yielded a more uniform distribution of particles with a smaller mean diameter (158 vs. 191渭m). SEM showed that the particles produced by grinding without salt precipitation were irregularly shaped, sheet-like particles. ECM particles produced by the salt precipitation method were round and porous in appearance with many particles in the range of 1渭m which tended to agglomerate with the larger particles and with each other. We conclude that the production of a comminuted form of ECM is possible and that the uniformity of particle size and shape are dependent upon the manufacturing methodology.
机译：研究了两种从猪膀胱中产生颗粒状细胞外基质（ECM）的方法。生产粉末的一种方法涉及将材料的冻干形式速冻，然后在研磨机中将其粉碎。第二种方法是相似的，只是在快速冷冻之前将ECM在NaCl溶液中饱和，然后在研磨前将盐晶体沉淀在基质中。几种方法被用来分析粒度分布和超微结构，包括声波筛分，激光衍射和扫描电子显微镜（SEM）。盐沉淀法可使平均直径较小的颗粒分布更均匀（158对191μm）。 SEM显示，通过研磨而没有盐沉淀的颗粒是不规则形状的片状颗粒。用盐沉淀法制得的ECM颗粒是圆形的，外观上是多孔的，许多颗粒在1μm的范围内，倾向于与较大的颗粒和彼此凝聚。我们得出的结论是，粉碎的ECM形式的生产是可能的，并且颗粒大小和形状的均匀性取决于制造方法。

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