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Aggregation effects due to aluminum adsorption to cell walls of the unicellular green alga Scenedesmus obtusiusculus

机译:铝吸附到单细胞绿藻Scendesmus obtusiusculus细胞壁上的聚集效应

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摘要

Cells of the unicellular green alga Scenedesmus obtusiusculus Chod. were cultivated for 2-24 h in nutrient media with low (60 mu mol/L) or high (1000 mu mol/L) phosphorus (P) concentration, and in the presence or absence of 222 mu mol/L aluminum chloride (Al). Cell aggregation was studied by using light microscope, sedimentation and centrifugation. After 2 h. Al was adsorbed to the cell surface and cell aggregates were formed by the attraction of the cells to each other. Aluminum is bound by the negative charges of the cell walls, and studies at different pH showed that a high proportion of positively charged Al forms promote cell aggregation. This effect was most pronounced in low phosphorus cultures as phosphate reduces the effect of Al on cell aggregation by forming aluminum-phosphate. Algae cultivated in the absence of Al did not show any cell aggregation tendencies.
机译:单细胞绿藻Scendesmus obtusiusculus Chod的细胞。在低(60μmol/ L)或高(1000μmol/ L)磷(P)浓度以及是否存在222μmol/ L氯化铝(Al)的营养培养基中培养2-24小时)。通过光学显微镜,沉淀和离心研究细胞聚集。 2小时后。 Al被吸附到细胞表面,并且由于细胞彼此的吸引而形成细胞聚集体。铝被细胞壁的负电荷所束缚,并且在不同pH值下的研究表明,高比例的带正电的Al形式促进细胞聚集。这种作用在低磷培养物中最为明显,因为磷酸盐通过形成磷酸铝减少了Al对细胞聚集的作用。在没有铝的情况下培养的藻类没有显示任何细胞聚集趋势。

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