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首页> 外文期刊>Biomaterials >Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems.
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Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems.

机译：用于基因传递系统的精氨酸嫁接生物可还原聚二硫胺。

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摘要

Arginine-grafted bioreducible poly(disulfide amine) (ABP) polymer was synthesized for non-viral gene delivery systems. Its Mw was measured to be 4.45x10(3) Da/mole by FPLC-SEC and its PDI value was 1.49. ABP was able to retard pDNA from a weight ratio of 2 but ABP could not retard pDNA even at a weight ratio of 10 in the presence of DTT, showing that it can be biodegraded in reducing environment such as cytoplasm. ABP was examined to form positively charged nano-sized particles (<200 nm) with pDNA. ABP showed no significant cytotoxicity and greatly enhanced transfection efficiency in comparison with unmodified poly(cystaminebisacrylamide-diaminohexane) (poly(CBA-DAH)) and PEI25k in mammalian cells. The transfection efficiency of ABP was not much reduced even in the serum condition. Chloroquine treatment was not found to improve the transfection efficiency of ABP. The cellular uptake pattern of ABP polyplexes was almost similar with poly(CBA-DAH), suggesting that greatly enhanced transfection efficiencyof ABP is not induced by its high cellular penetrating ability but may be mediated by other factors such as good nuclear localization ability.
机译：合成了精氨酸接枝的生物可还原聚二硫胺（ABP）聚合物用于非病毒基因传递系统。通过FPLC-SEC测定其Mw为4.45x10（3）Da / mole，其PDI值为1.49。在存在DTT的情况下，ABP能够从2的重量比延迟pDNA，但即使在10的重量比，ABP也不能延迟pDNA，这表明它可以在还原性环境（如细胞质）中被生物降解。检查了ABP与pDNA形成带正电的纳米级颗粒（<200 nm）。与未修饰的聚（胱胺双丙烯酰胺-二氨基己烷）（poly（CBA-DAH））和PEI25k在哺乳动物细胞中相比，ABP没有显示出明显的细胞毒性并大大提高了转染效率。即使在血清条件下，ABP的转染效率也不会降低很多。未发现氯喹处理可改善ABP的转染效率。 ABP复合物的细胞摄取模式与poly（CBA-DAH）几乎相似，这表明ABP转染效率的大幅提高不是由其高的细胞穿透能力引起的，而可能由其他因素（如良好的核定位能力）介导。

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