...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials >Immobilization of alkaline phosphatase on microporous nanofibrous fibrin scaffolds for bone tissue engineering.
【24h】

Immobilization of alkaline phosphatase on microporous nanofibrous fibrin scaffolds for bone tissue engineering.

机译：将碱性磷酸酶固定在用于骨组织工程的微孔纳米纤维蛋白纤维支架上。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Alkaline phosphatase (ALP) promotes bone formation by degrading inorganic pyrophosphate (PP(i)), an inhibitor of hydroxyapatite formation, and generating inorganic phosphate (P(i)), an inducer of hydroxyapatite formation. P(i) is a crucial molecule in differentiation and mineralization of osteoblasts. In this study, a method to immobilize ALP on fibrin scaffolds with tightly controllable pore size and pore interconnection was developed, and the biological properties of these scaffolds were characterized both in vitro and in vivo. Microporous, nanofibrous fibrin scaffolds (FS) were fabricated using a sphere-templating method. ALP was covalently immobilized on the fibrin scaffolds using 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC). Scanning electron microscopic observation (SEM) showed that mineral was deposited on immobilized alkaline phosphatase fibrin scaffolds (immobilized ALP/FS) when incubated in medium supplemented with beta-glycerophosphate, suggesting that the immobilized ALP was active. Primary calvarial cells attached, spread and formed multiple layers on the surface of the scaffolds. Mineral deposition was also observed when calvarial cells were seeded on immobilized ALP/FS. Furthermore, cells seeded on immobilized ALP/FS exhibited higher osteoblast marker gene expression compared to control FS. Upon implantation in mouse calvarial defects, both the immobilized ALP/FS and FS alone treated group had higher bone volume in the defect compared to the empty defect control. Furthermore, bone formation in the immobilized ALP/FS treated group was statistically significant compared to FS alone group. However, the response was not robust.
机译：碱性磷酸酶（ALP）通过降解无机焦磷酸盐（PP（i））（羟基磷灰石形成的抑制剂）并生成无机磷酸盐（P（i））（羟基磷灰石形成的诱导剂）来促进骨骼形成。 P（i）是成骨细胞分化和矿化的关键分子。在这项研究中，开发了一种将ALP固定在具有可控孔径和孔互连的血纤蛋白支架上的方法，并在体外和体内对这些支架的生物学特性进行了表征。使用球形模板法制备微孔，纳米纤维蛋白纤维支架（FS）。使用1-乙基-3-（二甲基氨基丙基）碳二亚胺盐酸盐（EDC）将ALP共价固定在血纤蛋白支架上。扫描电子显微镜观察（SEM）显示，当在添加了β-甘油磷酸的培养基中孵育时，矿物质沉积在固定的碱性磷酸酶纤维蛋白支架（固定的ALP / FS）上，表明固定的ALP具有活性。初级颅盖细胞附着，扩散并在支架表面形成多层。当颅骨细胞接种在固定的ALP / FS上时，也观察到矿物质沉积。此外，与对照FS相比，接种在固定化ALP / FS上的细胞表现出更高的成骨细胞标记基因表达。与空缺损对照相比，植入小鼠颅盖缺损后，固定化ALP / FS和单独FS治疗组的缺损骨量均更高。此外，与单独使用FS组相比，固定化ALP / FS治疗组的骨形成具有统计学意义。但是，响应并不强劲。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号