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首页> 外文期刊>Phytopathologia Mediterranea >Volatile metabolites associated with one aflatoxigenic and one nontoxigenic Aspergillus flavus strain grown on two different substrates
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Volatile metabolites associated with one aflatoxigenic and one nontoxigenic Aspergillus flavus strain grown on two different substrates

机译：与在两种不同底物上生长的一种黄曲霉毒素和一种非毒素黄曲霉菌株相关的挥发性代谢物

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摘要

Aflatoxigenic and non-toxigenic Aspergillus flavus strains were grown on corn and on peanut substrates. Microbial volatile organic compounds (MVOCs) were collected by trapping headspace volatiles using thermal desorption tubes (TDT) packed with Tenax (R) TA and Carbotrap (TM) B. Samples were collected at various fungal growth stages. Trapped compounds were thermally desorbed from the adsorbent tubes, separated by gas chromatography, and identified by mass spectrometry. The fungal stage did not have many differences in the MVOCs but the concentrations of some volatiles changed over time depending on the substrate. Volatiles that were associated with both the aflatoxigenic A. flavus strain and the nontoxigenic strain on both substrates included: ethanol, 1-propanol, butanal, 2-methyl-1-propanol, 3-methylfuran, ethyl acetate, 1-butanol, 3-methylbutanal, 3-methyl-1-butanol, propanoic acid-2-methyl-ethyl-ester, 2-methyl-1-butanol, 1-pentanol, 2-pentanol, 3-methyl-3-buten-1-ol, benzaldehyde, 3-octarione, 2-ethyl-1-hexanol and octane. Volatiles that were associated only with the aflatoxigenic A. flavus strain included: dimethyl disulfide and nonanal. Volatiles that were associated only with the nontoxigenic A. flavus strain included: hexanal, 1-hexanol, 1-octene-3-ol, 1-octen-3-one and 2-pentyl furan.
机译：黄曲霉毒素和非毒素黄曲霉菌株分别在玉米和花生基质上生长。通过使用装有Tenax（R）TA和Carbotrap（TM）B的热脱附管（TDT）捕获顶空挥发物，收集微生物挥发性有机化合物（MVOC）。在各种真菌生长阶段收集样品。将被捕集的化合物从吸附剂管中热脱附，通过气相色谱分离，并通过质谱鉴定。真菌阶段的MVOC差异不大，但某些挥发物的浓度随时间而变化，具体取决于底物。在两种底物上与黄曲霉黄曲霉菌株和非毒素菌株同时存在的挥发物包括：乙醇，1-丙醇，丁醛，2-甲基-1-丙醇，3-甲基呋喃，乙酸乙酯，1-丁醇，3-甲基丁醛，3-甲基-1-丁醇，丙酸-2-甲基-乙基酯，2-甲基-1-丁醇，1-戊醇，2-戊醇，3-甲基-3-丁烯-1-醇，苯甲醛，3-辛酮，2-乙基-1-己醇和辛烷。仅与产黄曲霉黄曲霉菌株相关的挥发物包括：二甲基二硫和壬醛。仅与非毒性黄曲霉菌株相关的挥发物包括：己醛，1-己醇，1-辛烯-3-醇，1-辛烯-3-酮和2-戊基呋喃。

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