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首页> 外文期刊>Phytopathologia Mediterranea >Studies on the susceptibility of pruning wounds to infection by fungi involved in grapevine wood diseases in Italy
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Studies on the susceptibility of pruning wounds to infection by fungi involved in grapevine wood diseases in Italy

机译:在意大利研究修剪伤口对真菌感染葡萄木材疾病的敏感性

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摘要

The susceptibility of grapevine annual pruning wounds to Phaeomoniella chlamydospora, Phaeoacremonium aleophilum and Diplodia seriata was investigated over three years (2005-2007) in a 15 year-old vineyard, cv. Sauvignon blanc. Vines were pruned each year in January, February and March and the wounds were inoculated weekly with conidial suspensions, and with sterile water as a control. Penetration of the fungi into the wood was assessed after 4 weeks by plating pieces of host tissue on agar medium. The susceptibility of annual pruning wounds, expressed as the infection percentages of inoculated spurs, varied with both the trial year and the fungus inoculated. Average infection percentages of inoculated spurs in the three years were respectively 14.7, 38.5 and 50.9% for Pa. chlamydospora, 31.7, 32.2 and 49.4% for Pm. aleophilum and 84.2, 43.8 and 40.9% for D. seriata. The period of pruning was significant for the infection percentages of all fungi in 2005, and for D. seriata in 2006. Natural infection of control spurs by Pa. chlamydospora (2, 4.4, and 11.7% of spurs in the three years respectively) and by Pm. aleophilum (0.3, 1.8, and 6.4%) began when average weekly temperatures stabilized around 10 degrees C, while inflection by D. seriata (12.2, 12 and 18.3% in the same period) occurred even below that threshold. Higher infection percentages of both artificially and naturally infected spurs in 2007 were probably due to the higher temperatures recorded in February and March (besides the use of a more efficient selective medium for the isolation of Pa. chlamydospora and Pm. aleophilum Only artificial infections with D. seriata showed an opposite trend that cannot be explained by the weather data. Infection of one-year-old wood appeared to be an important factor in disease spread. Spurs remained liable to infections with any of the fungi for up to 4 months after pruning, and isolation percentages could be fairly high also in late spring. As a consequence, the planning of pruning does not seem to be an effective means to counteract the wood diseases caused by these fungi.
机译:在三年(2005年至2007年)的一个具有15年历史的葡萄园中,调查了葡萄年度修剪伤口对衣原体Phaeomoniella chlamydospora,Phaeoacremonium aleophilum和Diplodia seriata的敏感性。长相思每年一月,二月和三月对葡萄树进行修剪,每周用分生孢子悬浮液和无菌水作为对照接种伤口。 4周后,通过将宿主组织碎片铺在琼脂培养基上,评估真菌向木材的渗透。年度修剪伤口的敏感性,以接种马刺的感染百分数表示,随试验年份和接种真菌的不同而不同。三年中,接种的马刺的平均感染百分率分别为:衣原体为14.7%,38.5%和50.9%,Pm为31.7%,32.2%和49.4%。嗜酒菌和D. seriata的84.2、43.8和40.9%。修剪期对2005年所有真菌的感染百分率和2006年对D. seriata的感染百分率都是重要的。沙眼衣原体对控制性马刺的自然感染(三年中分别占马刺的2%,4.4%和11.7%)和由Pm。当每周平均温度稳定在10摄氏度左右时,开始出现aleophilum(0.3%,1.8%和6.4%),而D. seriata(同期的12.2%,12%和18.3%)的拐弯甚至低于该阈值。 2007年人工和自然感染的马刺的感染百分比较高,可能是由于2月和3月记录的温度较高(除了使用更高效的选择性培养基分离了衣原体和嗜酸杆菌之外,仅D的人工感染。seriata显示出相反的趋势,无法用天气数据来解释;感染一岁的木材似乎是疾病传播的重要因素;修剪后的长达四个月之内,马刺仍容易感染任何真菌。 ,并且隔离率在春季末期也可能很高,因此,修剪计划似乎并不是抵抗这些真菌引起的木材疾病的有效手段。

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