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首页> 外文期刊>Photochemistry and Photobiology: An International Journal >Real-time visualization of photochemically induced fluorescence of 8-halogenated quinolones: Lomefloxacin, clinafloxacin and BAY3118 in live human HaCaT keratinocytes
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Real-time visualization of photochemically induced fluorescence of 8-halogenated quinolones: Lomefloxacin, clinafloxacin and BAY3118 in live human HaCaT keratinocytes

机译：实时可视化人类HaCaT角质形成细胞中8卤代喹诺酮类药物的光化学诱导荧光：洛美沙星，克林沙星和BAY3118

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摘要

Halogenoquinolones are potent and widely used antimicrobials blocking microbial DNA synthesis. However, they induce adverse photoresponses through the absorption of UV light, including phototoxicity and photocarcinogenicity. The phototoxic responses may be the result of photosensitization of singlet oxygen, production of free radicals and/or other reactive species resulting from photodehalogenation. Here, we report the use of laser scanning confocal microscopy to detect and to follow the fluorescence changes of one monohalogenated and three di-halogenated quinolones in live human epidermal keratinocyte cells during in situ irradiation by confocal laser in real time. Fluorescence image analysis and co-staining with the LysoTracker probe showed that lysosomes are a preferential site of drug localization and phototransformations. As the lysosomal environment is relatively acidic, we also determined how low pH may affect the dehalogenation and concomitant fluorescence. With continued UV irradiation, fluorescence increased in the photoproducts from BAY y3118 and clinafloxacin, whereas it decreased for lomefloxacin and moxifloxacin. Our images not only help to localize these phototoxic agents in the cell, but also provide means for dynamic monitoring of their phototransformations in the cellular environment.
机译：卤代喹诺酮类药物有效且广泛用于阻止微生物DNA合成。但是，它们通过吸收紫外线引起不良的光响应，包括光毒性和光致癌性。光毒性反应可能是单线态氧的光敏化，自由基的产生和/或由光脱卤作用产生的其他反应性物质的结果。在这里，我们报告使用激光扫描共聚焦显微镜实时检测并跟踪在活人表皮角质形成细胞中原核照射过程中活的人表皮角质形成细胞中一个单卤代和三个二卤代喹诺酮的荧光变化。荧光图像分析和与LysoTracker探针共染色显示，溶酶体是药物定位和光转化的优先位点。由于溶酶体环境是相对酸性的，因此我们还确定了低pH值可能会影响脱卤和伴随的荧光。随着持续的紫外线照射，BAY y3118和clinafloxacin的光产物中的荧光增加，而洛美沙星和莫西沙星则减少。我们的图像不仅有助于将这些光毒剂定位在细胞中，而且还提供了在细胞环境中动态监控其光转化的手段。

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