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首页> 外文期刊>Phytopathology >Contribution of Erwinia amylovora Exopolysaccharides Amylovoran and Levan to Biofilm Formation: Implications in Pathogenicity
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Contribution of Erwinia amylovora Exopolysaccharides Amylovoran and Levan to Biofilm Formation: Implications in Pathogenicity

机译：淀粉欧文氏菌外多糖阿米洛芬和莱万对生物膜形成的贡献：致病性的影响。

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摘要

Erwinia amylovora is a highly virulent, necrogenic, vascular pathogen of rosaceous species that produces the exopolysaccharide amylovoran, a known pathogenicity factor, and levan, a virulence factor. An in vitro crystal violet staining and a bright-field microscopy method were used to demonstrate that E. amylovora is capable of forming a biofilm on solid surfaces. Amylovoran and levan production deletion mutants were used to determine that amylovoran was required for biofilm formation and that levan contributed to biofilm formation. In vitro flow cell and confocal microscopy were used to further reveal the architectural detail of a mature biofilm and differences in biofilm formation between E. amylovora wild-type (WT), ams, and lsc mutant cells labeled with green fluorescent protein or yellow fluorescent protein. Scanning electron microscopy analysis of E. amylovora WT cells following experimental inoculation in apple indicated that extensive biofilm formation occurs in xylem vessels. However, ams mutant cells were nonpathogenic and died rapidly following inoculation, and lsc mutant cells were not detected in xylem vessels and were reduced in movement into apple shoots. These results demonstrate that biofilm formation plays a critical role in the pathogenesis of E. amylovora.
机译：淀粉欧文氏小球藻是蔷薇属物种的高毒性，坏死性，血管病原体，可产生已知的致病性因子胞外多糖amylovoran和致病性因子levan。体外结晶紫染色和明场显微镜方法被用来证明支链淀粉菌能够在固体表面上形成生物膜。使用阿米罗福兰和levan生产缺失突变体来确定阿米罗福兰是生物膜形成所必需的，并且levan有助于生物膜的形成。使用体外流动细胞和共聚焦显微镜进一步揭示了成熟生物膜的结构细节以及用绿色荧光蛋白或黄色荧光蛋白标记的支链淀粉野生型（WT），ams和lsc突变细胞之间生物膜形成的差异。 。在苹果中进行实验接种后，对淀粉小肠埃希氏菌WT细胞的扫描电子显微镜分析表明，木质部血管中发生了广泛的生物膜形成。但是，ams突变细胞是非致病性的，并且在接种后迅速死亡，并且在木质部血管中未检测到lsc突变细胞，并且向苹果芽的运动减少了。这些结果证明生物膜的形成在支链淀粉的发病机理中起着关键作用。

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