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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >Pathogenic bacteria can produce exopolysaccharides and use them as carbon source under stress conditions: the case of Erwinia amylovora
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Pathogenic bacteria can produce exopolysaccharides and use them as carbon source under stress conditions: the case of Erwinia amylovora

机译：致病细菌可以产生外核糖，并在压力条件下用作碳源：Erwinia氨基莫拉的案例

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摘要

Bacterial exopolysaccharides (EPSs) have long been related with cell protection under adverse conditions, but this information is still scarce in phytopathogenic bacteria. Erwinia amylovora, causal agent of the devastating fire blight disease, has to face with several stresses in plant environment such as starvation and toxic copper ions. Therefore, we aimed to study whether amylovoran, its major and most studied EPS, could be used as nutrient source by starved E. amylovora cells in presence of copper. Then, we monitored the culturability and the amylovoran levels of a wild type strain in comparison with its amylovoran-deficient mutant throughout six months in carbon-free AB medium plus 0.005 mM Cu~(2+), versus AB plus copper supplemented with 0.2% of amylovoran extract. A significant proportion of non-culturable E. amylovora cells was induced by starvation and copper stresses, but it was remarkably reduced by the use of amylovoran as nutrient in both the wild type strain and the amylovoran-deficient mutant. This phenomenon had not been previously reported either in plant pathogenic bacteria or in presence of copper.
机译：细菌脱核（EPS）长期与细胞保护在不利条件下有关，但这种信息仍然缺乏植物疗法细菌。 Erwinia Amylovora，毁灭性火焰疾病的因果因子，必须面对植物环境中的几种应力，如饥饿和有毒的铜离子。因此，我们旨在研究氨基洛兰，其主要和最研究的EPS是否可以用铜的氟化物淀粉虫细胞用作营养源。然后，在无碳AB培养基中加上0.005mM Cu〜（2+）的六个月，我们监测了野生型菌株的培养性和野生型菌株的培养性和淀粉醇水平，而ab加上铜加上0.2％淀粉醇提取物。通过饥饿和铜应力诱导显着比例的非培养物E.淀粉醇细胞，但在野生型菌株和缺氧缺陷型突变体中使用淀粉醇作为营养物显着降低。此现象以前尚未在植物病原细菌或铜存在下报道。

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