Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor

Jiangtao Gao; Song Qin; Yicheng Zhang

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Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor

机译：在搅拌罐式生物反应器中快速优化转基因海带紫菜配子体细胞培养工艺条件的优化

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Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH.The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor.Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 deg C and 200rpm.Optimal incident light intensities depended on the initial cell densities.

机译：在在线控制的5 L搅拌罐光生物反应器中研究了转基因海藻配子体细胞的分批培养，以通过监测pH的升高速度来快速优化工艺条件。转基因海带配子体具有编码乙型肝炎表面抗原（HBsAg）的异源基因配子体的最佳反应温度为15℃和200rpm。最佳入射光强度取决于初始细胞密度。

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《Chemical engineering journal》 |2006年第2期|共4页
作者
Jiangtao Gao; Song Qin; Yicheng Zhang;
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正文语种 eng
中图分类化学工业;
关键词
Gametophyte cells; HBsAg; Laminaria japonica; pH; Process conditions; Stirred-tank bioreactor;

机译：配子体细胞;HBsAg;海带;pH;工艺条件;搅拌槽生物反应器;

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1. Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor [J] . Jiangtao Gao, Song Qin, Yicheng Zhang Chemical engineering journal . 2006,第1a2期

机译：在搅拌罐式生物反应器中快速优化转基因海带紫菜配子体细胞培养工艺条件的优化
2. Optimal light regime for the cultivation of transgenic Laminaria japonica gametophytes in a bubble-column bioreactor [J] . Jiangtao Gao, Yicheng Zhang, Wei Zhang, Biotechnology Letters . 2005,第18期

机译：在气泡柱生物反应器中培养转基因海带紫菜配子体的最佳光照方案
3. Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor. [J] . Deng XiangYuan, Qin Song, Zhang Qun, Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants . 2009,第3期

机译：日本海带配子体的微粒轰击和转基因株系在气泡柱生物反应器内的快速繁殖。
4. Photolithotrophic Cultivation of Laminaria japonica Gametophyte Cells in Bioreactor [C] . International symposium on progress and prospect of marine biotechnology . 1999

机译：BioreActor中Laminaria japonica配饰细胞的光型营养培养
5. Productivity studies utilizing recombinant CHO cells in stirred-tank bioreactors: A comparative study between the pitch-blade and packed-bed bioreactor systems. [D] . Hatton, Taylor S. 2012

机译：利用搅拌罐式生物反应器中的重组CHO细胞进行生产力研究：沥青桨叶和填充床生物反应器系统之间的比较研究。
6. Trait evaluation and trial cultivation of Dongfang No. 2 the hybrid of a male gametophyte clone of Laminaria longissima (Laminariales Phaeophyta) and a female one of L. japonica [O] . Xiaojie Li, Yizhou Cong, Guanpin Yang, -1

机译：东方2号配子体克隆的Laminaria longissima（LaminarialesPhaeophyta）和女性L. japonica的杂交组合东方2号的性状评估和试验栽培
7. Optimal light regime for the cultivation of transgenic Laminaria japonica gametophytes in a bubble-column bioreactor [O] . JT Gao, YC Zhang, SJ Wu, 2005

机译：在气泡柱生物反应器中培养转基因海带紫菜配子体的最佳光照方案

Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor

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