首页> 美国政府科技报告 >Translation of Hepatitis A Virus RNA In vitro: Aberrant Internal InitiationsInfluenced by 5' Noncoding Region

【24h】

Translation of Hepatitis A Virus RNA In vitro: Aberrant Internal InitiationsInfluenced by 5' Noncoding Region

机译：体外甲型肝炎病毒RNa的翻译：由5'非编码区影响的异常内部启动

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Hepatitis A virus (HAV) RNAs were translated in vitro in rabbit reticulocytelysates. The pattern of proteins synthesized from full-length HAV RNA was highly complex, consisting of a continuous spectrum of polypeptides. The pattern was not significantly altered by varying incubation times, ion, or other reaction parameters, or by the addition of HeLa or BS-C-1 cell extracts to the translation reactions. Plasmids engineered with mutations in the 3C coding region produced transcripts which directed the synthesis of the same overall pattern of polypeptide products as those transcribed from wild-type sequences, suggesting that protein processing by 3C did not generate the complex set of protein products. Translation of RNA containing only the P3 coding region of HAV, directly adjacent to the HAV 5' noncoding region, generated a set of protein products which precisely matched a subset of those synthesized from full-length HAV RNA. The translation products of P3 RNA, full-length RNA, and mutant 3C-containing RNAs were analyzed by immunoprecipitation with antisera specific for 3D, VP1, and 2C sequences; several products were subjected to N-terminal sequence analysis.

著录项

作者
Jia, X. Y.; Scheper, G.; Brown, D.; Updike, W.; Harmon, S.;
展开▼
作者单位

展开▼
年度 1991
页码
总页数 12
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Antibodies; Continuous spectra; Hepatitis; Immune serums; Immunoassay; In vitroanalysis; Incubation; Mutations; Parameters; Patterns; Peptides; Plasmids; Polymers; Precipitation; Proteins; Response; Ribonucleic acids; Synthesis; Viruses; Reprints;

机译：抗体;连续光谱;肝炎;免疫血清;免疫分析;体外分析;孵育;突变;参数;模式;肽;质粒;聚合物;沉淀;蛋白质;反应;核糖核酸;合成;病毒;重印;

相似文献

外文文献
中文文献
专利

1. Roles of the polypyrimidine tract and 3' noncoding region of hepatitis C virus RNA in the internal ribosome entry site-mediated translation. [J] . Wang H, Shen XT, Ye R, Archives of virology . 2005,第6期

机译：丙型肝炎病毒RNA的多嘧啶束和3'非编码区在内部核糖体进入位点介导的翻译中的作用。
2. The La antigen binds 5' noncoding region of the hepatitis C virus RNA in the context of the initiator AUG codon and stimulates internal ribosome entry site-mediated translation [J] . Naushad Ali, Aleem Siddiqui Proceedings of the National Academy of Sciences of the United States of America . 1997,第6期

机译：La抗原在启动子AUG密码子的背景下结合丙型肝炎病毒RNA的5'非编码区，并刺激内部核糖体进入位点介导的翻译
3. THE LA ANTIGEN BINDS 5' NONCODING REGION OF THE HEPATITIS C VIRUS RNA IN THE CONTEXT OF THE INITIATOR AUG CODON AND STIMULATES INTERNAL RIBOSOME ENTRY SITE-MEDIATED TRANSLATION [J] . Ali N., Siddiqui A. Proceedings of the National Academy of Sciences of the United States of America . 1997,第6期

机译：在启动子Aug密码子的背景下，LA抗原与肝病毒C病毒RNA的5'非编码区结合，并刺激内部核糖体进入位点介导的翻译
4. Identification of a cellular protein required for hepatitis C virus internal ribosome entry site (IRES)-mediated translation [C] . T. PENG, H. TANG, F. WONG-STAAL Falk Symposium . 2004

机译：鉴定丙型肝炎病毒内部核糖体入口部位（IRES）介导的翻译所需的细胞蛋白质
5. Translational control of the hepatitis C virus RNA genome by an unusual internal ribosome entry site. [D] . Lancaster, Alissa Michele. 2005

机译：丙型肝炎病毒RNA基因组的翻译控制由异常的内部核糖体进入位点引起。
6. Cytoplasmic Expression of mRNAs Containing the Internal Ribosome Entry Site and 3′ Noncoding Region of Hepatitis C Virus: Effects of the 3′ Leader on mRNA Translation and mRNA Stability [O] . Li Kuo Kong, Peter Sarnow 2002

机译：包含丙型肝炎病毒内部核糖体进入位点和3非编码区的mRNA的胞质表达：3前导序列对mRNA翻译和mRNA稳定性的影响
7. Cytoplasmic Expression of mRNAs Containing the Internal Ribosome Entry Site and 3′ Noncoding Region of Hepatitis C Virus: Effects of the 3′ Leader on mRNA Translation and mRNA Stability [O] . Kong, Li Kuo, Sarnow, Peter 2002

机译：包含丙型肝炎病毒内部核糖体进入位点和3'非编码区的mRNA的胞质表达：3'前导序列对mRNA翻译和mRNA稳定性的影响

Translation of Hepatitis A Virus RNA In vitro: Aberrant Internal InitiationsInfluenced by 5' Noncoding Region

摘要

著录项

相似文献

相关主题

期刊订阅