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Biological Activity and Structural Stability of N-Deglycosylated RecombinantHuman Erythropoietin

机译：N-去糖基化重组人促红细胞生成素的生物活性和结构稳定性

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Controversy exists regarding the functional role of N-linked oligosaccharides inthe hormone erythropoietin. We have now examined the role of carbohydrates in the hormone's action using quantitative enzymatic deglycosylation. N-deglycosylated hormone exhibited full biological activity and potency in vitro. Denaturing with 6M urea and renaturing revealed that both the native and N-deglycosylated forms recovered full activity as long as the intrachain disulfide bonds remained intact. Therefore, receptor recognition, subsequent biological activity and maintenance of tertiary structure are intrinsic properties of the polypeptide chain of erythropoietin.

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Stykowski, A. J.; Feldman, L.; Zurbuch, D. J.;
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年度 1991
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总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Erythropoietins; Biology; Bonding; Carbohydrates; Hormones; In vitro analysis; Potency; Recognition; Sense organs; Stability; Structural properties; Sulfides; Reprints;

机译：促红细胞生成素;生物学;结合;碳水化合物;激素;体外分析;效力;识别;感觉器官;稳定性;结构特性;硫化物;重印;

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Biological Activity and Structural Stability of N-Deglycosylated RecombinantHuman Erythropoietin

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