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首页> 美国政府科技报告 >Discrimination of Pathogenic vs. Nonpathogenic Francisella tularensis and Burkholderia pseudomallei Using Proteomics Mass Spectrometry
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Discrimination of Pathogenic vs. Nonpathogenic Francisella tularensis and Burkholderia pseudomallei Using Proteomics Mass Spectrometry

机译：利用蛋白质组学质谱鉴别病原性与非致病性土拉弗朗西斯菌和伯克霍尔德氏菌的鉴别

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The recent use of mass spectrometry (MS) analysis to characterize and identify biological agents using a proteomic approach has been demonstrated. The present study sought to determine whether proteomics MS could be used to distinguish between pathogenic and nonpathogenic strains of the same organism based on outer membrane proteins (OMPs). OMPs are often associated with virulence in gram-negative pathogens and could prove to be excellent model biomarkers for strain differentiation among bacteria. OMPs were isolated from pathogenic and nonpathogenic strains of Francisella tularensis and Burkholderia pseudomallei using ultracentrifugation and a sarkosyl extraction method. These methods are followed by protein digestion and analysis using liquid chromatography-MS/MS. The experimental MS/MS spectral data of bacterial peptides were searched using the SEQUEST algorithm against a constructed proteome database of microorganisms to determine the identity and number of unique peptides. Results showed that pathogenic strains could be distinguished from nonpathogenic strains for both organisms based on differences in OMP profiles. Virulent F. tularensis and B. pseudomallei expressed multiple OMPs that were not present in avirulent samples. In conclusion, proteomics MS proved useful in discriminating between pathogenic and non-pathogenic strains, and OMPs unique to pathogenic strains could prove to be useful biomarkers for strain differentiation.

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