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How Alterations in the Cdt1 Expression Lead to Gene Amplification in Breast Cancer

机译：Cdt1表达的改变如何导致乳腺癌的基因扩增

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We examined the effects of Cdt1 for gene amplification by using inducible system. We Obtained and validated reagents for inducible Cdt1- expression-system. Furthermore, we identified appropriated dose of MTX for testing gene amplification Cdt2 is a key molecule for inhibiting Cdt1 activity. The depletion of Cdt2 induces DNA re-replication in several cell lines. During experiment, we Identified a reduction of PCNA monoubiquitination in Cdt2 depleted MCF7 cells.Exogenous Cdt2 rescued the effect of si-Cdt2. Mutant of Cdt2 indicated that binding Cdt2 to Cul4/DDB1 was required for PCNA monoubiquitination. Furthermore, we identified that CRL4Cdt2 complex promotes PCNA-dependent translesion DNA synthesis.Additionally, we have identified p12 as a novel target of CRL4 complex. Cdt2 depletion canceled p12 degradation which regulated checkpoint pathway. Stable form of p12 promotes fork progression during intra-S-phase checkpoint.

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作者
Terai, K.;
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年度 2011
页码 1-29
总页数 29
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Breast cancer ; Amplification ; Cells(Biology) ; Chemical agents ; Deoxyribonucleic acids ; Depletion ; Genes ; Mutations ; Reduction ; Targets ; Validation;

机译：乳腺癌;扩增;细胞（生物学）;化学试剂;脱氧核糖核酸;耗尽;基因;突变;减少;靶标;验证;

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1. HER-2 protein overexpressing breast cancer without gene amplification shows higher hormone receptor expression than HER-2 protein overexpressing breast cancer with gene amplification. [J] . Koo JS, Jung W, Yang WI International journal of surgical pathology . 2011,第4期

机译：没有基因扩增的HER-2蛋白过表达的乳腺癌比具有基因扩增的HER-2蛋白过表达的乳腺癌显示更高的激素受体表达。
2. High-level amplification at 17q23 leads to coordinated overexpression of multiple adjacent genes in breast cancer [J] . J P?rssinen, T Kuukasj?rvi, R Karhu, British Journal of Cancer . 2007,第8期

机译：17q23的高水平扩增导致乳腺癌中多个相邻基因的协同过表达
3. Human epidermal growth factor receptor 1 (EGFR) expression was not associated with gene amplification but intimately assoclated with HER2 gene amplification and protein expression in tissue microarray of clinical breast cancers [J] . S.K.Ahn, Gwak, K.Park, European Journal of Cancer Supplements . 2006,第2期

机译：人表皮生长因子受体1（EGFR）的表达与基因扩增无关，但与临床乳腺癌组织芯片中的HER2基因扩增和蛋白质表达密切相关
4. Combination of gene expression and genome copy number alteration has a prognostic value for breast cancer [C] . Cava, C., Zoppis, I., Mauri, G., Annual Conference of Japanese Society for Medical and Biological Engineering;Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2013

机译：基因表达和基因组拷贝数改变的结合对乳腺癌有预后价值
5. Modulation of plant gene expression using antisense technology and gene amplification: Alteration of shikimate pathway gene expression. [D] . Jones, James Dale. 1994

机译：使用反义技术和基因扩增调节植物基因表达：sh草酸酯途径基因表达的改变。
6. High-level amplification at 17q23 leads to coordinated overexpression of multiple adjacent genes in breast cancer [O] . J Pärssinen, T Kuukasjärvi, R Karhu, 2007

机译：17q23处的高水平扩增导致乳腺癌中多个相邻基因的协同过表达
7. High-level amplification at 17q23 leads to coordinated overexpression of multiple adjacent genes in breast cancer [O] . Pärssinen, J, Kuukasjärvi, T, Karhu, R, 2007

机译：17q23处的高水平扩增导致乳腺癌中多个相邻基因的协同过表达

How Alterations in the Cdt1 Expression Lead to Gene Amplification in Breast Cancer

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