Development and Testing of Recombinant Single Domain Antibodies

机译：重组单结构域抗体的开发和测试

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Conventional antibodies, widely used in detection schemes, such point detectors for biological threats, are large 150 kDa proteins that aggregate irreversibly under harsh conditions such as excessive heating. There is a need for the development of high affinity, highly specific robust recognition elements to overcome the limitations of conventional antibodies. One option is single domain antibodies (sdAb), the molecularly engineered binding elements derived from camelids and shark. SdAb are small 12-16 kDa robust recognition elements able to function after exposure to chemical denaturants and elevated temperatures. These antibody fragments contain only 3 hypervariable loops but can still bind with affinity equivalent to conventional antibody fragments having twice the number of complementarity-determining regions (CDRs) available to interact with the target. We developed and characterized sdAb towards toxins (ricin, abrin and SEB) and viral (filovirus) targets. We employed a combination of strategies, including sandwich immunoassays, surface plasmon resonance, circular dichroism, mutagenesis, and crystallography to help us to understand the mechanism of this bio-molecular recognition and provide critical insights to the basis of the sdAb-target interaction and sdAb properties.

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Goldman, E.; Hayhurst, A.;
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年度 2010
页码 1-16
总页数 16
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Biological detection ; Antibodies ; Biological agent detectors ; Immunoassay ; Ricin ; Recognition;

机译：生物检测;抗体;生物制剂检测器;免疫测定;蓖麻毒素;识别;

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3. In Silico Analysis Of The Structure Of Variable Domains Of Mouse Single-chain Antibodies Specific To The Human Recombinant Interferon β1b [J] . A. I. Flyak, M. V. Pavlova, P. V. Gilchuk Cytology and genetics . 2009,第1期

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Development and Testing of Recombinant Single Domain Antibodies

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