• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国政府科技报告 >Formation of Lignans(-)-Secoisolariciresinol and (-)-Matairesinol with Forsythia intermedia Cell=Free Extracts
【24h】

Formation of Lignans(-)-Secoisolariciresinol and (-)-Matairesinol with Forsythia intermedia Cell=Free Extracts

机译：木质素（ - ） - secoisolariciresinol和（ - ） - matairesinol与连翘中间细胞=自由提取物的形成

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vivo labeling experiments of Forsythia intermedia plant tissue with (8-(C-14))- and (9,9-(2)H2,OC(2)H3)coniferyl alcohols revealed that the lignans, (-)-secoisolariciresinol and (-)-matairesinol, were derived from two coniferyl alcohol molecules; no evidence for the formation of the corresponding (+)-enantiomers was found. Administration of (+/-)-(Ar-(H-3)) secoisolariciresinols to excised shoots of F.intermediaresulted in a significant conversion into (-)-matairesinol; again, the (+)-antipode was not detected. Experiments using cell-free extracts of F.intermedia confirmed and extended these findings. In the presence of NAD(P)H and H2O2, the cell-free extracts catalyzed the formation of (-)-secoisolariciresinol, with either (8-(C-14))- or (9,9-(2)H2,OC(2)H3)coniferyl alcohols as substrates. The (+)- enantiomerwas not formed. Finally, when either (-)-(Ar-(H-3)) or (+/-)-(Ar-(H-2)) secoisolariciresinols were used as substrates, in the presence of NAD(P), only (-)- and not (+)-matairesinol formation occurred. The other antipode, (+)-secoisolariciresinol, did not serve asa substrate for the formation of either (+)- or (-)-matairesinol. Thus, in F.intermedia, the formation of the lignan, (-)-secoisolariciresinol, occurs under strict stereochemical control, in a reaction or reactions requiring NAD(P)H and H2O2 as cofactors. This stereoselectivity is retained in the subsequent conversion into (-)-matairesinol, since (+)-secoisolariciresinol is not a substrate. These are the first two enzymes to be discovered in lignan formation.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号