• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Effects of cooling time on membrane integrity and motility of frozen-thawed canine spermatozoa using two different commercial egg yolk–based extenders at two different cooldown equilibration times
【2h】

Effects of cooling time on membrane integrity and motility of frozen-thawed canine spermatozoa using two different commercial egg yolk–based extenders at two different cooldown equilibration times

机译：在两种不同的冷却平衡时间下，使用两种不同的基于蛋黄的扩展剂，冷却时间对冻融犬精子膜完整性和运动性的影响

页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two commercially available egg yolk–based semen extenders, one marketed for human semen freezing (HEYE) and one marketed for canine semen freezing (CEYE), were used to cryopreserve semen from single ejaculates of 11 different dogs. For each extender, a 30- and a 60–min cooldown period was used prior to the addition of the extender containing glycerol and then immediately frozen in liquid nitrogen vapours. Sperm motility was measured using a computer-assisted semen analysis (CASA) system. Sperm intact membranes were measured using SYBER-14 and propidium iodide. Semen in the HEYE cooled for 60 min had a significantly greater percentage of intact membranes than the semen in the HEYE cooled for 30 min (p = 0.02). Semen in the HEYE cooled for 60 min had significantly greater total motility (p = 0.007) and progressive motility (p = 0.004) than semen cooled for 60 min in the CEYE and semen cooled for 30 min in the HEYE (total motility p = 0.02 and progressive motility p = 0.02). Semen cooled for 60 min in the CEYE did not differ significantly in total (p = 0.6) or progressive motility (p = 0.4) than semen cooled for 30 min in the CEYE. There was no difference in total (p = 0.8) or progressive motility (p = 0.8) between the semen cooled for 30 min in the HEYE and the semen cooled for 30 min in the CEYE.
机译：有两种市售的基于蛋黄的精液补充剂，一种用于人精液冷冻（HEYE），另一种用于犬精液冷冻（CEYE），用于冷冻来自11只不同狗的单次射精的精液。对于每种增量剂，在添加含甘油的增量剂之前，需要分别进行30分钟和60分钟的冷却，然后立即在液氮蒸气中冷冻。使用计算机辅助精液分析（CASA）系统测量精子活力。使用SYBER-14和碘化丙啶测量完整的精子膜。冷却60分钟的HEYE精液中完整膜的百分比明显高于冷却30分钟的HEYE精液（p = 0.02）。在HEYE中冷却60分钟的精液的总运动性（p = 0.007）和进行性运动（p = 0.004）显着高于在CEYE中冷却60 min的精液和HEYE中冷却30 min的精液（总运动性p = 0.02）和进行性运动p = 0.02）。在CEYE中冷却60分钟的精液与在CEYE中冷却30分钟的精液相比，总体（p = 0.6）或进行性运动（p = 0.4）没有显着差异。在HEYE中冷却30分钟的精液与CEYE中冷却30分钟的精液之间的总剂量（p = 0.8）或进行性运动（p = 0.8）没有差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号