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The molecular characterisation, strain selection and induction of lipid synthesis in a suitable marine microalgal species for potential biodiesel production

机译：用于潜在生物柴油生产的合适海洋微藻物种的分子表征，菌株选择和脂质合成的诱导

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The demand for alternative energy has increased exponentially and algal-based biofuels are viewed as a viable alternative. This study examined the lipid producing capability of Isochrysis and the role of phosphorus depletion in stimulating lipid production. The main objective of this study was to determine the relationship between varying levels of phosphorus depletion and total lipid production in a suitable marine microalga. The ability of the microalgal strain to grow and produce lipid were examined under varying concentrations of phosphorus in the f/2 medium. Isochrysis exhibited log growth after 8 days of cultivation in the various dilutions of the phosphorus concentration recommended for f/2 medium. A stationary phase was observed when the microalgal cells depleted the surrounding medium of phosphorus. The phosphorus concentration of the various media was measured using a phosphorus colorimetric assay which revealed that the phosphorus concentration of the media progressively decreased from day 2 to day 24. However, there was no further uptake of phosphorus from the surrounding medium when the cells entered a stationary phase. The medium containing no phosphorus supplementation stimulated a lipid production of 57.75 % after 24 days which was attributed to the microalgae accumulating lipid under phosphorus deprivation. This was a result of continuous lipid synthesis combined with a low rate of cell division and photosynthesis. Therefore, allowing Isochrysis to deplete the medium of soluble reactive phosphorus resulted in increased lipid production. Furthermore, a relationship between phosphorus deprivation and lipid content was attained for sustained lipid production over time.
机译：对替代能源的需求呈指数增长，基于藻类的生物燃料被视为可行的替代品。这项研究检查了等渗线虫的脂质生产能力以及磷消耗在刺激脂质生产中的作用。这项研究的主要目的是确定合适的海洋微藻中磷耗竭的不同水平与总脂质产量之间的关系。在f / 2培养基中磷浓度不同的情况下，检查了微藻菌株生长和产生脂质的能力。在推荐用于f / 2培养基的各种磷浓度稀释液中培养8天后，等渗线表现出对数增长。当微藻细胞耗尽了周围的磷培养基时，观察到一个固定相。使用磷比色法测量了各种培养基中的磷浓度，结果表明，从第2天到第24天，培养基中的磷浓度逐渐降低。但是，当细胞进入细胞中时，周围的磷不再吸收。固定相。不添加磷的培养基在24天后刺激了57.75％的脂质产生，这归因于微藻在缺磷的情况下积累了脂质。这是连续脂质合成与低细胞分裂和光合作用结合的结果。因此，使等渗线耗尽可溶的反应性磷的培养基导致脂质产生增加。此外，随着时间的推移持续产生脂质，磷缺乏与脂质含量之间存在关系。

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    Nambiar Shari;

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  • 年度 2011
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