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首页> 外文OA文献 >Discovery of a Suppressor of ADF1 and the Mapping of a new ADF gene, ADF2, in Chlamydomonas reinhardtii
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Discovery of a Suppressor of ADF1 and the Mapping of a new ADF gene, ADF2, in Chlamydomonas reinhardtii

机译：在莱茵衣藻中发现ADF1抑制剂并绘制新的ADF基因ADF2的图谱

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摘要

Eukaryotic cilia are evolutionary conserved microtubule-based organelles that play important roles in cell signalling, development and motility. Cilia can be shed through a process known as deflagellation, in which the cilium is severed at a specific site at its base in response to a stress signal, through calcium signalling. Fifteen years ago three genes were uncovered in a screen for deflagellation mutants, two of which were cloned and their roles in the microtubule-severing event characterized. The third gene, named ADF1 has to date eluded identification. A previous graduate student genetically mapped ADF1 to Linkage Group (Chromosome) IX to identify the ADF1 gene. I tried, unsuccessfully, to rescue the adf1 mutation with wild type DNA from the gene locus. In the rescue attempts I recovered a potential suppressor of the adf1-3 mutant. Despite the many attempts I was unable to rescue adf1 or identify the flanking DNA of the adf1-3 suppressor. Since no MT severing protein or calcium sensor were recovered in the original genetic screen a second genetic screen is being carried out, this time for conditional deflagellation mutants. From this screen I characterized a new acid induced deflagellation gene, ADF2, and mapped it to Linkage Group III. The work in this thesis thus contributes new information about two previously unknown deflagellation genes, ADF2 and ADF1-Suppressor.
机译：真核纤毛是进化保守的基于微管的细胞器，在细胞信号传导，发育和运动中起重要作用。纤毛可以通过称为脱鞭毛的过程脱落，在该过程中，纤毛在钙基信号响应的压力信号下，在其基部的特定部位被切断。十五年前，在爆燃突变体的筛选中发现了三个基因，其中两个被克隆并表征了它们在微管切断事件中的作用。迄今为止，尚未鉴定出第三个名为ADF1的基因。以前的一名研究生将ADF1遗传定位到Linkage Group（Chromosome）IX，以鉴定ADF1基因。我尝试从基因座中拯救野生型DNA的adf1突变，但未成功。在救援尝试中，我找到了adf1-3突变体的潜在抑制因子。尽管进行了许多尝试，但我仍无法挽救adf1或鉴定adf1-3抑制剂的侧翼DNA。由于在原始遗传筛选中未恢复MT分离蛋白或钙传感器，因此正在进行第二次遗传筛选，这一次是针对有条件的脱鞭毛突变体。在这个屏幕上，我对一个新的酸诱导的脱鞭毛基因ADF2进行了鉴定，并将其定位到Linkage Group III。因此，本论文的工作为有关两个以前未知的减速基因ADF2和ADF1-Suppressor提供了新的信息。

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    Rodriguez Julie Coralina;

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  • 年度 2013
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