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首页> 外文OA文献 >Concomitant Retrograde Coronary Venous Infusion of Basic Fibroblast Growth Factor Enhances Engraftment and Differentiation of Bone Marrow Mesenchymal Stem Cells for Cardiac Repair after Myocardial Infarction.
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Concomitant Retrograde Coronary Venous Infusion of Basic Fibroblast Growth Factor Enhances Engraftment and Differentiation of Bone Marrow Mesenchymal Stem Cells for Cardiac Repair after Myocardial Infarction.

机译：伴随的逆行冠状静脉输注碱性成纤维细胞生长因子促进骨髓间充质干细胞移植和分化心肌梗死后的心脏修复。

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AIM: Basic fibroblast growth factor (bFGF) increases the migration and viability of bone marrow mesenchymal stem cells (MSCs) in vitro. Retrograde coronary venous infusion can provide both increased regional bFGF concentrations and homogeneous cell dissemination. We determined whether retrograde delivery of bFGF enhances the potency of transplanted MSCs for cardiac repair in a canine infarct model.METHODS AND RESULTS: Under hypoxic conditions, cellular migration was significantly increased in MSCs co-cultured with bFGF compared to vascular endothelial growth factor or insulin-like growth factor, and bFGF promoted MSCs differentiation into a cardiomyocyte phenotype. A canine infarct model was employed by coronary ligation. One week later, animals were subjected to retrograde infusion of combination bFGF (200ng/mL) and MSCs (1×10(8) cells) (n=5), MSCs (1×10(8) cells, n=5), bFGF (200ng/mL, n=5), or placebo (phosphate-buffered saline, n=3). Four weeks after infusion, only the bFGF+MSCs therapy exhibited significantly increased left ventricular ejection fraction (LVEF) by echocardiography (pCONCLUSIONS: Retrograde coronary venous bFGF infusion augments engraftment and differentiation capacity of transplanted MSCs, recovering cardiac function and preventing adverse remodeling. This novel combined treatment and delivery method is a promising strategy for cardiac repair after ischemic injury.
机译：目的：碱性成纤维细胞生长因子（bFGF）在体外可增加骨髓间充质干细胞（MSC）的迁移和活力。逆行冠状静脉输注可以提供增加的区域bFGF浓度和均匀的细胞扩散。我们确定了bFGF的逆向递送是否增强了移植的MSC在犬梗死模型中的心脏修复能力。方法和结果：在缺氧条件下，与bFGF共培养的MSCs与血管内皮生长因子或胰岛素相比，细胞迁移显着增加。类生长因子和bFGF促进MSCs分化为心肌细胞表型。通过冠状动脉结扎术使用犬梗塞模型。一周后，对动物进行bFGF（200ng / mL）和MSCs（1×10（8）细胞）（n = 5），MSCs（1×10（8）细胞，n = 5）的逆行输注， bFGF（200ng / mL，n = 5）或安慰剂（磷酸盐缓冲液，n = 3）。输注后四周，只有bFGF + MSCs治疗通过超声心动图检查显示左心室射血分数（LVEF）显着增加（p结论：逆行冠状静脉bFGF输注可增强移植的MSC的植入和分化能力，恢复心脏功能并防止不良重塑。联合治疗和分娩方法是缺血性损伤后心脏修复的有前途的策略。

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