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首页> 外文OA文献 >The effect of potential large-scale bioreactor environmental heterogeneities during fed-batch culture on the performance of an industrially-relevant GS-CHO cell culture, producing an IgG antibody
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The effect of potential large-scale bioreactor environmental heterogeneities during fed-batch culture on the performance of an industrially-relevant GS-CHO cell culture, producing an IgG antibody

机译：潜在的大规模生物反应器环境异质性在补料分批培养中对工业相关Gs-CHO细胞培养物的性能的影响，产生IgG抗体

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摘要

This study aimed to study the effect of potential large-scale bioreactor environmental heterogeneities during fed-batch culture on the performance of an industrially-relevant GS-CHO cell culture, producing an IgG antibody. Heterogeneity was created by a two-compartment scale-down model, using a well-mixed stirred tank reactor (STR) and plug flow reactor (PFR). A peristaltic pump was used to continuously circulate cell culture from the STR through the PFR. Standard culture parameters were measured and flow cytometry was used to indicate cell viability and mode of cell death.udThe results essentially fell into two categories: those without circulation and those with it. In all cases with recirculation, whether nutrients and alkali were added into the STR or the PFR, significantly decreased culture duration ((sim)48 hours shorter) and antibody titre ((sim)20% decrease) were found compared to those experiments without circulation.udIt was impossible to conclude anything concerning the impact of bioreactor heterogeneities. Nevertheless, damage associated with peristaltic pumping has relevance to the many aspects of cell culture processes that require transfer of cells in suspension. The 'squeezing' motion of peristaltic pumps may impose sufficient mechanical stress to have decreased cell culture performance. The high biocompatibility of the materials used suggests long-term incompatibility is less likely.ud
机译：这项研究旨在研究分批补料培养过程中潜在大规模生物反应器环境异质性对与工业相关的GS-CHO细胞培养物产生IgG抗体的性能的影响。通过使用均匀混合的搅拌釜反应器（STR）和活塞流反应器（PFR）的两室按比例缩小模型来创建异质性。使用蠕动泵使细胞培养物从STR通过PFR连续循环。测量标准培养参数，并使用流式细胞仪指示细胞活力和细胞死亡模式。 ud结果基本上分为两类：无循环的和有循环的。在所有再循环情况下，无论是在STR还是PFR中添加了营养和碱，都显着缩短了培养时间（缩短了48小时）和抗体滴度（降低了20％）。与没有循环的那些实验相比。 ud不可能得出任何有关生物反应器异质性影响的结论。然而，与蠕动泵送有关的损害与细胞培养过程的许多方面有关，这些过程需要悬浮细胞的转移。蠕动泵的“挤压”运动可能会施加足够的机械应力，从而降低细胞培养性能。使用的材料具有很高的生物相容性，表明长期不相容的可能性较小。 ud

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    Scott W H;

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  • 年度 2011
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  • 正文语种 {"code":"en","name":"English","id":9}
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