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首页> 外文OA文献 >Project 1: The biophysical effects of low intensity ultrasound on the morphology, growth and differentiation potential of bone marrow-derived Mesenchymal stem cells and Project 2: The dynamic changes in histone modifications on the Hoxb genes that accompany embryonic stem cell differentiation.
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Project 1: The biophysical effects of low intensity ultrasound on the morphology, growth and differentiation potential of bone marrow-derived Mesenchymal stem cells and Project 2: The dynamic changes in histone modifications on the Hoxb genes that accompany embryonic stem cell differentiation.

机译：项目1：低强度超声对骨髓间充质干细胞形态，生长和分化潜能的生物物理作用和项目2：伴随胚胎干细胞分化的Hoxb基因组蛋白修饰的动态变化。

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Project 1: udMesenchymal stem cells (MSCs) are considered to be an attractive target for cell-replacement therapies due to their extensive self-renewal and multipotent differentiation capabilities. Applying ultrasound to soft tissue lesions and bone fractures has been shown to promote repair of damaged tissue. It was suggested that ultrasound stimulates repair by activating bone-marrow derived MSCs (BMSCs) to begin proliferating and differentiating to replenish the tissue. Therefore, the aim of the study was to analyse the biophysical effects of low-frequency, low-intensity ultrasound on the differentiation and colony forming unit ability of BMSCs. To test this colony forming unit assays were performed on ultrasound treated and untreated cells and cell staining was used to analyse the effects. Furthermore, to determine the intracellular response to ultrasound stimulation, the expression of ultrasound-sensitive genes was also analysed by reverse-transcriptase PCR. The results obtained in this study showed that BMSCs treated with low-frequency, low-intensity ultrasound did not generate a greater number of colonies than untreated cells. Colony size was found to be unchanged by treatment. Furthermore, ultrasound treatment did not appear to enhance the differentiation potential of BMSCs down the osteogenic lineage. This data suggests that low-frequency, low-intensity ultrasound does not appear to activate BMSCs in culture. ududProject 2: udEmbryonic stem cells (ESCs) are a pluripotent cell population that are able to self-renew and differentiate into cell types of all three germ layers. ESCs are able to differentiate by maintaining developmental genes in a transcriptionally poised state. The Hox genes are developmental regulators which are arranged in clusters. During differentiation, the genes are sequentially expressed according to their position in the cluster. To determine whether changes in histone modifications on Hoxb genes during differentiation reflect changes in their expression, ESCs were differentiated and quantitative PCR was used to determine the patterning of Hoxb1, 5 and 9 expression. Native Chromatin Immunoprecipitation (NChIP) was performed to determine the enrichment of histone modifications. The data showed that changes in histone modifications on Hoxb1 and 5 correlated with the temporal and spatial patterning of expression. Hoxb9 expression did not correlate with its position in the cluster and the levels of histone modifications did not reflect the patterning of expression observed. Also the fold enrichment of marks on the Hoxb genes did not correspond with changes in levels on Hoxa genes. This shows that changes in histone modifications on Hoxb genes reflect their transcriptional status, however but were not predictive of gene expression.
机译：项目1： ud间充质干细胞（MSC）由于其广泛的自我更新和多能分化能力而被认为是细胞置换疗法的诱人靶标。已显示将超声应用于软组织病变和骨折可促进受损组织的修复。提示超声波通过激活源自骨髓的MSC（BMSC）开始增殖和分化以补充组织来刺激修复。因此，本研究的目的是分析低频，低强度超声对BMSCs分化和集落形成单位能力的生物物理影响。为了测试该菌落形成单位，对超声处理和未处理的细胞进行了测定，并使用细胞染色来分析其作用。此外，为了确定细胞对超声刺激的反应，还通过逆转录酶PCR分析了超声敏感基因的表达。在这项研究中获得的结果表明，低频，低强度超声处理的骨髓间充质干细胞没有比未处理的细胞产生更多的菌落。通过处理发现菌落大小不变。此外，超声治疗似乎并未增强成骨细胞系下骨髓间充质干细胞的分化潜能。该数据表明，低频，低强度超声似乎并未激活培养物中的BMSC。 ud ud项目2： ud胚胎干细胞（ESC）是一种多能细胞群，能够自我更新并分化为所有三个细菌层的细胞类型。 ESC能够通过将发育基因保持在转录平衡状态来进行区分。 Hox基因是成簇排列的发育调节因子。在分化期间，基因根据它们在簇中的位置顺序表达。为了确定在分化期间Hoxb基因上的组蛋白修饰的变化是否反映了它们的表达变化，对ESC进行了分化，并使用定量PCR确定了Hoxb1、5和9表达的模式。进行天然染色质免疫沉淀（NChIP）以确定组蛋白修饰的富集。数据显示，Hoxb1和5的组蛋白修饰的变化与表达的时间和空间模式有关。 Hoxb9表达与其在簇中的位置不相关，组蛋白修饰的水平也不反映观察到的表达模式。同样，Hoxb基因上标记的富集倍数与Hoxa基因上水平的变化不符。这表明Hoxb基因上组蛋白修饰的变化反映了它们的转录状态，但是不能预测基因表达。

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    Munir Hafsa;

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  • 年度 2012
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