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AFLP Fingerprinting reveals genetic variability in common carp stocks from Indonesia

机译:aFLp指纹图谱揭示了印度尼西亚鲤鱼种群的遗传变异

摘要

Amplified Fragment Length Polymorphism (AFLP) fingerprinting was used to detect geneticudvariability in two stocks of common carps (Majalaya carp and Sinyonya carp) and secondudgeneration gynogens derived from the Majalaya carp. Primer combinations, E-AAC/M-CAA, EAGG/udM-CAC and E-ACC/M-CTT yielded 299 selectively amplified loci of which 66.2% wereudpolymorphic. Maximum polymorphism was evident in the Majalaya carp and minimum amongudthe gynogens. Four types of AFLP bands which were useful to differentiate the stocks in questionudwere identified. Band Sharing Index (BSI) matrix was generated from the band binaryudvalue sets. Maximum average BSI was exhibited by the gynogens and minimum by theudMajalaya carp. Inter-stock comparisons showed the gynogens to be closest to the Majalaya andudfarthest from the Sinyonya stock.
机译:扩增片段长度多态性(AFLP)指纹图谱用于检测两种普通鲤科鱼类(Majalaya鲤鱼和Sinyonya鲤鱼)和源自Majalaya鲤鱼的第二代或后代雌雄激素的遗传/变异性。引物组合,E-AAC / M-CAA,EAGG / udM-CAC和E-ACC / M-CTT产生299个选择性扩增的基因座,其中66.2%是多态的。在马加拉亚in鱼中,最大多态性是明显的,在雌雄激素中则最小。确定了四种类型的AFLP波段,这些波段可用于区分所讨论的股票。波段共享索引(BSI)矩阵是根据波段二进制 udvalue集生成的。雌孕激素表现出最大的平均BSI,而 udMajalaya鲤鱼表现出最小的BSI。种群之间的比较显示,雌雄激素与马贾拉亚邦最接近,距辛尼扬亚种群最远。

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