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首页> 外文期刊>Asian Fisheries Science >AFLP fingerprinting reveals genetic variability in common carp stocks from indonesia
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AFLP fingerprinting reveals genetic variability in common carp stocks from indonesia

机译：AFLP指纹图谱揭示了印度尼西亚鲤鱼种群的遗传变异

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摘要

Amplified Fragment Length Polymorphism (AFLP) fingerprinting was used to detect genetic variability in two stocks of common carps (Majalaya carp and Sinyonya carp) and second generation gynogens derived from the Majalaya carp. primer combinations, E-AAC/M-CAA, E-AGG/M-CAC and E-ACC/M-CTT yielded 299 selectively amplified loci of which 66.2% were polymorphic. Maximum polymorphism was evident in the Majalaya carp and minimum among the gynogens. Four types of AFLP bands which were useful to differentiate the stocks in question were identified. Band sharing Index (BSI) matrix was generated from the band binary value sets. Maximum average BSI was exhibited by the gynogens and minimum by the Majalaya carp. Inter-stock comparisons showed the gynogens to be closest to the Majalaya and farthest from the Sinyonya stock.
机译：扩增片段长度多态性（AFLP）指纹图谱用于检测两种普通鲤科鱼类（Majalaya鲤鱼和Sinyonya鲤鱼）和源自Majaalaya鲤鱼的第二代雌激素原的遗传变异性。引物组合E-AAC / M-CAA，E-AGG / M-CAC和E-ACC / M-CTT产生299个选择性扩增的基因座，其中66.2％为多态性。最大的多态性在Majaalaya鲤鱼中明显，而在雌激素中则最小。确定了四种类型的AFLP频段，这些频段可用于区分相关存货。从频带二进制值集生成频带共享索引（BSI）矩阵。雌孕激素表现出最大的平均BSI，而Majalaya鲤鱼表现的最小。种群之间的比较显示，雌雄激素最接近马加拉亚邦，而距信永家种群最远。

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