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鉴定孟氏隐唇瓢虫的特异性DNA序列、引物、鉴定方法及鉴定试剂盒

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摘要

本发明公开了一种鉴定孟氏隐唇瓢虫的特异性DNA序列、引物、鉴定方法及鉴定试剂盒。该鉴定孟氏隐唇瓢虫的特异性DNA序列如SEQ ID NO.1所示，其鉴定引物如SEQ ID NO.2和3所示。本发明克服了传统瓢虫分类对标本完整性要求高、需要专业分类专家、鉴定成本高等问题，提供的PCR引物及鉴定方法不仅能提高孟氏隐唇瓢虫鉴定的效率和准确性，而且操作简便快捷，适合于田间检测粉蚧类害虫种群中是否混有捕食性的孟氏隐唇瓢虫，对室内养殖过程进行质量监控、对孟氏隐唇瓢虫的生防效果进行准确评估等方面，具有重要的理论意义和应用价值。

著录项

公开/公告号CN103409536B

专利类型发明专利
公开/公告日2014-12-31

原文格式PDF
申请/专利权人广东省昆虫研究所;中山大学;
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申请/专利号CN201310360552.9
发明设计人张宇宏;庞虹;余彦莹;毛润乾;郑基焕;
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申请日2013-08-16
分类号C12Q1/68(20060101);C12N15/11(20060101);
代理机构44001 广州科粤专利商标代理有限公司;
代理人刘明星
地址 510260 广东省广州市海珠区新港西路105号
入库时间 2022-08-23 09:23:07

法律信息

法律状态公告日

法律状态信息

法律状态
2016-11-23

专利权人的姓名或者名称、地址的变更 IPC(主分类):C12Q 1/68 变更前: 变更后: 申请日:20130816

专利权人的姓名或者名称、地址的变更
2014-12-31

授权

授权
2013-12-18

实质审查的生效 IPC(主分类):C12Q 1/68 申请日:20130816

实质审查的生效
2013-11-27

公开

公开

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1. 鉴定孟氏隐唇瓢虫的特异性DNA序列、引物、鉴定方法及鉴定试剂盒 [P] . 中国专利： CN103409536B . 2014.12.31
2. 龟纹瓢虫的特异性分子标记DNA序列、鉴别引物、鉴定方法及鉴定试剂盒 [P] . 中国专利： CN103667446A . 2014-03-26
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机译：用于鉴定生物样品中优良事件，确认种子纯度，在存在所述优良事件的情况下选择种子的方法。用于确定植物，植物或植物的齐哥状态的状态包括所述事件精英的种子，以检测所述事件精英的存在。并且用于生产包含所述事件精英的植物或棉花种子，以及用于鉴定所述事件精英的试剂盒。适用于特异性检测的引物的鉴定以及用于鉴定所述事件精英的特异性探针
4. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

机译：油菜中与CMS ogura的Rfo恢复基因偶联的被测DNA片段末端区域特异的引物的核苷酸序列，通过基因组DNA片段的特异性引物与CMS ogura的Rfo基因偶联的聚合酶链反应（PCR）扩增DNA的方法在油菜中，制备与油菜CMS ogura的Rfo恢复子基因偶联的DNA片段的核苷酸序列的方法，在SCAR型DNA标记物的制备方法中用于鉴定含ogura基因的Rfo恢复子基因的油菜植物雄性不育，带有Rfo恢复基因的油菜植株的ogura基因胞质雄性不育的鉴定方法和试剂盒
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机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途