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METHOD FOR PREDICTING DISEASE SEVERITY AND OUTCOME IN PATIENTS WITH SURGICAL SEPSIS
METHOD FOR PREDICTING DISEASE SEVERITY AND OUTCOME IN PATIENTS WITH SURGICAL SEPSIS
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机译：外科脓毒症患者疾病严重程度和预后的预测方法
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摘要
FIELD: medicine.;SUBSTANCE: present invention relates to medicine, namely to diagnosing systemic inflammation and predicting severity and outcome of a disease in patients with surgical sepsis. For this, venous blood of patients are sampled in the first 48 hours after diagnosing sepsis from a peripheral vein into a test tube, K3EDTA is added. No more than 2 hours after blood sampling, an expression level of C3aR is assessed quantitatively on monocytes by the average fluorescence intensity on a flow cytometer. No less than 104 cells are analyzed in each sample, further in the course of treatment, blood is sampled again, expression level of C3aR is determined on monocytes and a prognostic index IC3aR1–4 is calculated in the disease dynamics according to the formula: IC3aR1 = N2 / N1, IC3aR2 = N3 / N1, IC3aR3 = N4 / N1, IC3aR4 = N5 / N1,where IC3aR1 – a prognostic index with accuracy of one decimal place in the disease dynamics, IC3aR2–4 – a prognostic index in the disease dynamics, N1 – an initial value of an expression index on C3aR monocytes (MFI) at the initial stage of the disease in the first 48 hours, N2–5 – an expression value on C3aR monocytes (MFI) in subsequent studies in the disease dynamics. If IC3aR value is greater than 1.0 and IC3aR2–4 increases with each subsequent study, dismal prognosis of disease severity and disease outcome in patients with surgical sepsis leading to death is made.;EFFECT: use of the claimed invention allows predicting the disease severity and outcome in patients with surgical sepsis.;1 cl, 3 ex
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机译：发明领域本发明涉及医学，即诊断外科性败血症患者的全身炎症并预测疾病的严重性和结果。为此，在诊断出脓毒症后的前48小时内应从患者的静脉血取样，将败血病从试管中抽出，并添加K 3 EDTA。采血后不超过2小时，通过流式细胞仪上的平均荧光强度定量评估C3aR在单核细胞上的表达水平。每个样品中分析的细胞不少于10个 4 ，在治疗过程中，再次采样血液，确定单核细胞上C3aR的表达水平，并预测预后指标IC3aR 1-4 是根据以下公式在疾病动态中计算的：IC3aR 1 = N 2 / N 1 ，IC3aR 2 = N 3 / N 1 ，IC3aR 3 = N 4 / N 1 ，IC3aR 4 = N 5 / N 1 ，其中IC3aR 1 –预后指标疾病动力学中小数点后的准确性IC3aR 2-4 –疾病动力学中的预后指标N 1 – C3aR单核细胞表达指标的初始值（MFI）在疾病最初的48小时内，N 2-5 –在随后的疾病动力学研究中，C3aR单核细胞（MFI）的表达值。如果随后的每项研究的IC3aR值均大于1.0，且IC3aR 2-4 升高，则对导致败血症的手术败血症患者死亡的疾病严重程度和疾病结局预后不良。要求保护的发明可以预测患有外科败血症的患者的疾病严重程度和预后。1cl，3 ex
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