首页> 外国专利> OLIGONUCLEOTIDE, SET OF OLIGONUCLEOTIDES, METHOD FOR SIMULTANEOUS DETECTION OF NEISSERIA MENINGITIDIS, STREPTOCOCCUS PNEUMONIAE AND HAEMOPHILUS INFLUENZAE, AND KIT

OLIGONUCLEOTIDE, SET OF OLIGONUCLEOTIDES, METHOD FOR SIMULTANEOUS DETECTION OF NEISSERIA MENINGITIDIS, STREPTOCOCCUS PNEUMONIAE AND HAEMOPHILUS INFLUENZAE, AND KIT

机译:寡核苷酸,寡核苷酸组,同时检测脑膜炎奈瑟菌,肺炎链球菌和流感嗜血杆菌和试剂盒的方法

摘要

The present invention provides a real-time PCR method which allows, in a single step, simultaneous detection of causative agents of bacterial meningitis, more specifically, Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. To this end, primers were developed that are used to amplify particular regions of the genomes of said bacteria. The presence of the bacteria in a sample is indicated by the presence of the amplicon, which is detected by detection methods that are suitable for the PCR method used.
机译:本发明提供了实时PCR方法,其允许在单个步骤中同时检测细菌性脑膜炎的病原体,更具体地,检测脑膜炎奈瑟氏球菌,肺炎链球菌和流感嗜血杆菌。为此,开发了用于扩增所述细菌基因组的特定区域的引物。样品中细菌的存在通过扩增子的存在来指示,该扩增子通过适合于所用PCR方法的检测方法进行检测。

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