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oligonucleotide, oligonucleotide pool, method for simultaneous detection of neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae, and, kit.
oligonucleotide, oligonucleotide pool, method for simultaneous detection of neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae, and, kit.
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机译:寡核苷酸,寡核苷酸池,同时检测脑膜炎奈瑟菌,肺炎链球菌和流感嗜血杆菌的方法和试剂盒。
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摘要
The present invention provides a real-time pcr method that allows in a single step simultaneous detection of etiological agents of bacterial meningitis, more specifically neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae. To this end, primers used to amplify particular regions of the genomes of said bacteria have been developed. The presence of bacteria in a sample is indicated by the presence of amplicon, which is detected by detection methods appropriate to the pcr methodology employed.
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