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Method for Determing Scrub Typhus Using measurement Interferon-Gamma Secretion of Peripheral Blood Mononuclear Cells
Method for Determing Scrub Typhus Using measurement Interferon-Gamma Secretion of Peripheral Blood Mononuclear Cells
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机译:测定外周血单个核细胞干扰素-γ分泌的测定斑疹伤寒的方法
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摘要
The present invention relates to a diagnostic method for scrub typhus using interferon- (IFN-) secretion measurement of peripheral blood mononuclear cells (PBMC), and more particularly, to a method for diagnosing Tsutsugamushi disease Induced IFN- production was increased by treating the extract of Tsutsugamushi extract with blood containing peripheral blood mononuclear cells or peripheral blood mononuclear cells isolated from patients infected with Tsutsugamushi bacteria, whereas IFN- production was increased in peripheral blood mononuclear cells- production. Therefore, it is possible to diagnose Tsutsugamushi disease simply by measuring the concentration of IFN-y production. Further, the method for diagnosing Tsutsugamushi disease of the present invention can be used for indirect immunofluorescence And the PCR analysis for genotype analysis, the accuracy of the diagnosis is remarkably improved, and the conventional diagnosis method Not only can you not completely replaced thereby significantly improving the reliability of the diagnosis.
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