首页> 外国专利> Method for Determing Scrub Typhus Using measurement Interferon-Gamma Secretion of Peripheral Blood Mononuclear Cells

Method for Determing Scrub Typhus Using measurement Interferon-Gamma Secretion of Peripheral Blood Mononuclear Cells

机译:测定外周血单个核细胞干扰素-γ分泌的测定斑疹伤寒的方法

摘要

The present invention relates to a diagnostic method for scrub typhus using interferon- (IFN-) secretion measurement of peripheral blood mononuclear cells (PBMC), and more particularly, to a method for diagnosing Tsutsugamushi disease Induced IFN- production was increased by treating the extract of Tsutsugamushi extract with blood containing peripheral blood mononuclear cells or peripheral blood mononuclear cells isolated from patients infected with Tsutsugamushi bacteria, whereas IFN- production was increased in peripheral blood mononuclear cells- production. Therefore, it is possible to diagnose Tsutsugamushi disease simply by measuring the concentration of IFN-y production. Further, the method for diagnosing Tsutsugamushi disease of the present invention can be used for indirect immunofluorescence And the PCR analysis for genotype analysis, the accuracy of the diagnosis is remarkably improved, and the conventional diagnosis method Not only can you not completely replaced thereby significantly improving the reliability of the diagnosis.
机译:本发明涉及一种使用干扰素(IFN-)分泌测量外周血单个核细胞(PBMC)来擦洗斑疹伤寒的诊断方法,更具体地,涉及一种用于诊断Tsu虫病的方法,该方法通过处理提取物而增加了诱导的IFN-产生。用含有gam虫病细菌感染的患者分离的外周血单核细胞或外周血单核细胞的血液提取Tsu虫提取物,而外周血单核细胞产生的IFN-产生增加。因此,可以仅通过测量IFN-γ产生的浓度来诊断Tsu虫病。此外,本发明的Tsu虫病的诊断方法可以用于间接免疫荧光,并且PCR分析用于基因型分析,显着提高了诊断的准确性,并且常规的诊断方法不仅不能完全替代,因此可以显着改善。诊断的可靠性。

著录项

  • 公开/公告号KR101809136B1

    专利类型

  • 公开/公告日2017-12-15

    原文格式PDF

  • 申请/专利权人 인하대학교 산학협력단;

    申请/专利号KR20150189371

  • 发明设计人 이진수;

    申请日2015-12-30

  • 分类号G01N33/68;C07K16/24;C12N5/0783;G01N15/14;G01N33/543;

  • 国家 KR

  • 入库时间 2022-08-21 12:41:25

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