首页> 外国专利> A SOLID NUTRIENT MEDIA USEFUL FOR ISOLATING AND IDENTIFYING ALKALIPHILIC BACTERIA

A SOLID NUTRIENT MEDIA USEFUL FOR ISOLATING AND IDENTIFYING ALKALIPHILIC BACTERIA

机译:固体营养培养基,可用于分离和鉴定碱性细菌

摘要

The present invention relates to a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form, wherein the media composition consisting of 5-15g of carbon source, 2.5-10g of peptone, 2.5-10g of yeast extract, 0.5-1.5g of dipotassium hydrogen phosphate; 0.1-0.5g of magnesium sulphate heptahydrate, 30µl-4ml of super saturated solution of sodium hydroxide, 5-20g of potassium chloride and 10-30g of K-carrageenan in one litre of distilled water. The present invention further provides a process for the preparation of a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form. The present invention also provides a method of using the solid nutrient media composition having alkaline pH to study biodiversity of cultivable alkaliphilic bacteria. The potassium salt in combination with K-carrageenan in specific proportion has been found to be a suitable replacement of agar in solidifying bacteriological media especially, for isolation of extreme alkaliphiles. The microbial techniques for purifying colonies by streaking can be carried out successfully using K-carrageenan as a gelling agent.
机译:具有碱性pH的固体营养培养基组合物本发明涉及具有碱性pH的固体营养培养基组合物,用于分离和鉴定纯净形式的嗜碱微生物,其中所述培养基组合物由5-15g碳源,2.5-10g蛋白,、 2.5-10g酵母提取物组成,0.5-1.5g磷酸氢二钾;一升蒸馏水中含0.1-0.5g七水合硫酸镁,30μl-4ml氢氧化钠超饱和溶液,5-20g氯化钾和10-30g K-角叉菜胶。本发明进一步提供了一种制备具有碱性pH的固体营养培养基组合物的方法,该组合物可用于分离和鉴定纯净形式的嗜碱微生物。本发明还提供了一种使用具有碱性pH的固体营养培养基组合物来研究可培养的嗜碱细菌的生物多样性的方法。已经发现钾盐与K-角叉菜胶以特定比例结合,特别是用于分离极端亲碱性的物质,是在凝固细菌培养基中琼脂的合适替代品。通过使用角叉菜胶作为胶凝剂,可以成功地进行通过划线纯化菌落的微生物技术。

著录项

  • 公开/公告号IN298711B

    专利类型

  • 公开/公告日2018-07-13

    原文格式PDF

  • 申请/专利权人

    申请/专利号IN58/DEL/2008

  • 申请日2008-01-07

  • 分类号C12N9/2417;

  • 国家 IN

  • 入库时间 2022-08-21 12:51:27

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