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Method for increasing citric acid production by Aspergillus niger fermentation

机译:黑曲霉发酵增加柠檬酸产量的方法

摘要

The invention discloses a method for improving citric acid production by Aspergillus niger fermentation, which integrates Aspergillus niger GABA pathway succinate semialdehyde dehydrogenase SSD gene into Aspergillus niger genome to obtain recombinant Aspergillus niger strain, and uses recombinant black The Aspergillus strain ferments to produce citric acid; the expression of the succinate semialdehyde dehydrogenase SSD gene is regulated by the Pgas promoter. The method of the invention realizes the expression of succinate semialdehyde dehydrogenase SSD in Aspergillus niger to enhance the GABA pathway so as to strengthen the TCA cycle and promote the synthesis of citric acid.
机译:本发明公开了一种黑曲霉发酵改善柠檬酸生产的方法,该方法将黑曲霉GABA途径的琥珀酸半醛脱氢酶SSD基因整合到黑曲霉中。基因组获得重组黑曲霉菌株,并用重组黑曲霉发酵产生柠檬酸。琥珀酸半醛脱氢酶SSD基因的表达受Pgas启动子调控。本发明方法实现了琥珀酸半醛脱氢酶SSD在黑曲霉中的表达,从而增强了GABA途径,从而增强了TCA循环,促进了柠檬酸的合成。

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