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Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis
Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis
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机译：细胞观察下的细胞液捕获及其成分分析以及用于细胞液捕获和分析的仪器
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摘要
A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.
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