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Method and kit for determining genomic integrity and / or quality of a library of DNA sequences obtained by whole genome amplification of definitive restriction enzyme sites
Method and kit for determining genomic integrity and / or quality of a library of DNA sequences obtained by whole genome amplification of definitive restriction enzyme sites
The present invention comprises: (a) providing a library of DNA sequences; and (b) PGR using at least one first primer pair, one second primer pair, and one third primer pair. A step of amplifying a library of DNA sequences, wherein said primer pair has a length of 1000 bp to 5000 bp and is located in each of the first chromosomal arm, the second chromosomal arm and the third chromosomal arm; A first PCR product, a second PCR product, and a third PCR product, each of which hybridizes to the DNA sequence of the library corresponding to the sequence, and wherein the amplification step is 50 bp to 1000 bp each and has a different size from each other. (C) detecting a first PCR product, a second PCR product, and a third PCR product; and (d) a first P product. Associating the presence of the R product, the second PCR product, and the third PCR product with the genomic integrity of the sample and / or the quality of the library of DNA sequences, and It relates to a method for determining the quality of a DNA sequence library obtained by definitive restriction enzyme site whole genome amplification (DRS-WGA) of the genome of a sample. [Selection] Figure 1
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