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METHOD FOR PRODUCING CHITIN AND CHITOSAN FROM GREEN BAY MUSSEL (PERNA VIRIDIS) SHELLS
METHOD FOR PRODUCING CHITIN AND CHITOSAN FROM GREEN BAY MUSSEL (PERNA VIRIDIS) SHELLS
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机译：用绿湾贻贝壳制造甲壳质和壳聚糖的方法
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摘要
A method for producing chitin and chitosan from green mussel (Perna viridis) shell comprising the steps of: cleaning green bay mussel shells thoroughly with distilled water; drying said green bay mussel shells in a controlled dryer at 35-40oC 'for 1 hour; pulverizing said dried green bay mussel shells until powder consistency using a pulverizer with 2mm mesh screen; soaking said powdered green bay mussel shells with 8 pcnt hydrochloric acid (1:12 w/v) for 1 hour at room temperature; straining said powdered green mussel shells from the solution using a filter funnel; washing said powdered green mussel shells with distilled water until a neutral pH is obtained; straining said washed powdered green bay mussel shells using a filter funnel to remove excess water; heating said powdered green bay mussel with 8 pcnt sodium hydroxide solution at 80-100oC in boiling water bath until dried to produce chitin; washing said chitin with distilled water until a neutral pH is obtained; drying said chitin under the heat of the sun for two (2) hours; bleaching said chitin with 3 pcnt sodium hypochlorite solution for 5 minutes; washing said chitin with distilled water; straining said chitin using a filter funnel to remove excess water; drying said chitin under the heat of the sun for two (2) hours; heating said chitin with 50 pcnt sodium hydroxide (1:12 w/v) in boiling water bath until dried to produce chitosan; washing said chitosan with distilled water until neutral pH is obtained; drying said chitosan under the heat of the sun for one (1) hour.
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机译：一种从绿贻贝壳生产甲壳质和壳聚糖的方法，包括以下步骤：用蒸馏水彻底清洗绿湾贻贝壳;将所述的绿湾贻贝壳在35-40℃的受控干燥器中干燥1小时;使用带有2mm筛目的粉碎机将所述干燥的绿湾贻贝壳粉碎至粉末稠度;在室温下，将所述粉状的绿湾贻贝壳用8％盐酸（1∶12w / v）浸泡1小时;使用过滤漏斗将所述粉状的绿色贻贝贝壳从溶液中滤出;用蒸馏水洗涤所述粉状的绿色贻贝贝壳，直到获得中性pH。用过滤漏斗过滤所述洗涤过的粉状的绿湾贻贝贝壳，以除去多余的水;在沸水浴中，于80-100oC下，将上述粉状绿贝贻贝与8 pcnt氢氧化钠溶液一起加热，直到干燥以产生几丁质。用蒸馏水洗涤所述几丁质直到获得中性pH。在阳光下将所述几丁质干燥两（2）小时;用3％次氯酸钠溶液漂白所述几丁质5分钟。用蒸馏水洗涤所述几丁质;使用滤漏斗过滤所述几丁质以除去过量的水;在阳光下将所述几丁质干燥两（2）小时;在沸水浴中将所述几丁质与50％的氢氧化钠（1:12 w / v）一起加热直至干燥以产生壳聚糖。用蒸馏水洗涤所述壳聚糖直至获得中性pH。将所述壳聚糖在阳光下干燥一（1）小时。
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