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Kit and method for measuring the activity of deoxynucleoside kinase by using DNA-dependent DNA polymerase AND NATURAL deoxynucleosides
Kit and method for measuring the activity of deoxynucleoside kinase by using DNA-dependent DNA polymerase AND NATURAL deoxynucleosides
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机译：利用DNA依赖性DNA聚合酶和天然脱氧核苷测定脱氧核苷激酶活性的试剂盒和方法
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摘要
The present invention relates to a kit comprising a DNA-dependant DNA polymerase and at least one natural deoxynucleoside and to a kit comprising a DNA-dependent DNA polymerase and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being displaced from, or bound to, dsDNA synthesized by said DNA-dependent polymerase. It further relates to A method for measuring deoxynucleoside kinase activity in a sample characterized by; (i) contacting the sample, in a container, with a reaction mix comprising a DNA-dependent DNA polymerase, at least one deoxynucleoside and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being incorporated in, displaced from, or bound to dsDNA synthesized by said DNA dependent polymerase; (ii) incubating said container; (iii) measuring the signal from the fluorescent moiety; and correlating the signal from the fluorescent moiety to the deoxynucleoside kinase activity in the sample.
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