首页> 外国专利> Chromogenic enzyme substrate, use of the same, culture medium and / or revelation, a process for the detection and / or identification and / or quantification of microorganisms expressing at least one peptidase activity, a process for differentiation between bacteria, among those belonging to the mi Cru00f3bios gram positive or gram negative and process for differentiation of yeast

Chromogenic enzyme substrate, use of the same, culture medium and / or revelation, a process for the detection and / or identification and / or quantification of microorganisms expressing at least one peptidase activity, a process for differentiation between bacteria, among those belonging to the mi Cru00f3bios gram positive or gram negative and process for differentiation of yeast

机译：发色酶底物，其使用，培养基和/或启示，用于检测和/或鉴定和/或定量表达至少一种肽酶活性的微生物的过程，用于区分细菌的过程属于mi Cr u00f3bios革兰氏阳性或革兰氏阴性和酵母分化过程

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Chromogenic enzyme substrates that are 7-amino-phenoxazine derivatives (I) are new. Chromogenic enzyme substrates that are 7-amino-phenoxazine derivatives of formula (I). [Image] R 1and R 2hydrogen, 1-6C alkyl, halo, COOR', CONR'R'', sulfo, or sulfonamide, or together they complete a naphthalene ring or optionally substituted coumarin of formula (a); R 3and R 4hydrogen, halo, COOR', CONR'R'', sulfo or sulfonamide, or together complete a naphthalene ring of formula (b); R 5and R 6hydrogen, halo, COOR', CONR'R'' or 1-6C alkyl; R 7and R 8hydrogen, 1-6C alkyl, aralkyl, aryl, carboxyalkyl, carboxy or sulfo, or together complete a 4-6C ring; R 9hydrogen, bromo, chloro, benzoyl, carboxy or sulfo, but must be other than hydrogen if R 5is hydrogen; R' and R'' : hydrogen or 1-6C alkyl or together they complete a heterocycle containing one or more heteroatoms; A : at least one amino acid; X : blocking group or is absent; Provided that at least one of R 1/R 2and R 3/R 4forms a phenyl or optionally substituted coumarin ring, and when R 1/R 2form a coumarin ring, then R 3and R 4do not form a phenyl ring; R 6is hydrogen when R 1/R 2form a ring and is halo when R 1/R 2and R 3/R 4each form a benzene ring. [Image] [Image] Independent claims are also included for: (1) reaction medium that includes at least one (I), optionally also at least one other enzyme substrate (X) specific for an enzyme activity other than that detected by (I); (2) process for detecting, identifying and/or quantifying microorganisms that express at least one peptidase activity, using the medium of (1); (3) process for differentiating between Gram positive and negative bacteria, using a medium of (1) in which the (I) present includes an alanine residue; and (4) process for differentiating between Candida albicansand C. tropicalisor C. glabrata, using a medium of (1) in which the (I) present includes a proline residue.

机译：7-氨基-吩恶嗪衍生物（I）的发色酶底物是新的。发色酶底物是式（I）的7-氨基-苯恶嗪衍生物。 [图片] R 1和R 2氢，1-6C烷基，卤素，COOR'，CONR'R''，磺基或磺酰胺，或一起构成萘环或式（a）的任选取代的香豆素。 R 3和R 4氢，卤素，COOR'，CONR'R''，磺基或磺酰胺，或一起完成式（b）的萘环; R 5和R 6氢，卤素，COOR'，CONR'R''或1-6C烷基; R 7和R 8氢，1-6C烷基，芳烷基，芳基，羧基烷基，羧基或磺基，或一起形成4-6C环; R 9是氢，溴，氯，苯甲酰基，羧基或磺基，但如果R 5是氢，则必须不是氢。 R′和R″：氢或1-6C烷基，或它们一起构成一个含有一个或多个杂原子的杂环; A：至少一种氨基酸; X：阻止基团或不存在;只要R 1 / R 2和R 3 / R 4中的至少一个形成苯基或任选取代的香豆素环，并且当R 1 / R 2形成香豆素环时，则R 3和R 4不形成苯环;当R 1 / R 2形成环时，R 6是氢，当R 1 / R 2和R 3 / R 4各自形成苯环时，R 6是卤素。 [影像] [权利要求]还包括以下方面的独立权利要求：（1）反应介质，其包含至少一种对除（I）检测到的酶活性以外的酶活性具有特异性的酶（I），还任选地还包含至少一种其他酶底物（X）。）; （2）使用（1）的培养基检测，鉴定和/或定量表达至少一种肽酶活性的微生物的方法; （3）使用（1）的培养基（其中存在的（I）包括丙氨酸残基）来区分革兰氏阳性菌和阴性菌的方法; （4）使用（1）的培养基区分白色念珠菌和热带念珠菌和光滑念珠菌的方法，其中存在的（I）包括脯氨酸残基。

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公开/公告号BRPI0410269B1

专利类型
公开/公告日2015-06-09

原文格式PDF
申请/专利权人 BIOMÉRIEUX;
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申请/专利号BR2004PI10269
发明设计人 ARTHUR JAMES;JOHN PERRY;ANNETTE RIGBY;STEPHEN STANFORTH;
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申请日2004-05-13
分类号C07D265/36;C07D265/38;C07D498/04;C12Q1/04;C12Q1/37;
国家 BR
入库时间 2022-08-21 15:16:14

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