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Method for auxiliary identification of inbred line of wuzhishan miniature pig and its special primer
Method for auxiliary identification of inbred line of wuzhishan miniature pig and its special primer
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机译：五指山微型猪近交系的辅助鉴定方法及其专用引物
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摘要
A method for auxiliary identification of WZSP inbred line and its special primers are disclosed. The method may comprise: testing whether a deoxyribose nucleotide at site 1273 from 5′ end of genomic DNA comprising SEQ ID NO: 1 in a test pig is A or G, measuring whether the test pig genotype is GG, GA or AA wherein the test pig with GG genotype is a candidate for the WZSP inbred line and the test pigs with GA genotype and AA genotype are non-WZSP inbred line. The method can be applied to breed WZSP inbred line, which can be used to preliminarily screen all the pigs in test pig group, eliminate non-WZSP inbred line, find out a candidate WZSP inbred line and make a further confirmation in combination with other methods. The method and primers can also be used to judge whether a WZSP on market is counterfeiting or not.
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机译：公开了一种WZSP自交系的辅助鉴定方法及其特殊引物。该方法可以包括：在测试猪中测试从包含SEQ ID NO：1的基因组DNA的5'端起的第1273位位点处的脱氧核糖核苷酸是A还是G，测量该测试猪的基因型是GG，GA还是AA。具有GG基因型的猪是WZSP自交系的候选者，具有GA基因型和AA基因型的受试猪是非WZSP自交系。该方法可用于WZSP自交系的选育，可用于初步筛选试验猪群中的所有猪，淘汰非WZSP自交系，找出候选WZSP自交系并结合其他方法做进一步确认。 。该方法和引物还可用于判断市场上的WZSP是否为假冒产品。
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