首页> 外国专利> Neutrophil gelatinase-associated lipocalin (NGAL) protein isoforms enriched from urine and recombinant chinese hamster ovary (CHO) cells and related compositions, antibodies, and methods of enrichment, analysis and use

Neutrophil gelatinase-associated lipocalin (NGAL) protein isoforms enriched from urine and recombinant chinese hamster ovary (CHO) cells and related compositions, antibodies, and methods of enrichment, analysis and use

机译：从尿液和重组中国仓鼠卵巢（CHO）细胞中富集的中性粒细胞明胶酶相关脂钙蛋白（NGAL）蛋白亚型以及相关的成分，抗体以及富集，分析和使用方法

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摘要

A composition comprising neutrophil gelatinase-associated lipocalin (NGAL), which has been enriched from urine, has a molecular weight of about 24.9 kDa to about 25.9 kDa, and comprises a plurality of isoforms having isoelectric points (pIs) ranging from about 5.9 to about 9.1; a composition comprising NGAL, which has been enriched from recombinant Chinese hamster ovary (CHO) cells, has a molecular weight of about 25.9 kDa to about 27.9 kDa, and comprises a plurality of isoforms having pIs ranging from about 5.6 to about 9.1; a method of obtaining from urine a composition comprising a plurality of isoforms of NGAL, which method comprises enriching NGAL in urine without separating molecules based on charge; a method of obtaining from recombinant CHO cells a composition comprising a plurality of isoforms of NGAL, which method comprises enriching NGAL in a composition without separating molecules based on charge; and a method of analyzing NGAL isoforms enriched from urine or recombinant CHO cells comprising analyzing an enriched composition comprising NGAL isoforms by two-dimensional electrophoresis and Western blot.

机译：包含从尿中富集的中性粒细胞明胶酶相关脂钙蛋白（NGAL）的组合物，其分子量为约24.9 kDa至约25.9 kDa，并且包含具有等电点（pIs）为约5.9至约1.65的多种同工型。 9.1;一种包含NGAL的组合物，其已经从重组中国仓鼠卵巢（CHO）细胞中富集，分子量为约25.9kDa至约27.9kDa，并且包含多个pI为约5.6至约9.1的同工型;一种从尿液中获得包含多种NGAL同工型的组合物的方法，该方法包括在尿液中富集NGAL而无需基于电荷分离分子;从重组CHO细胞获得包含多种NGAL同工型的组合物的方法，该方法包括在组合物中富集NGAL而无需基于电荷分离分子;一种分析从尿液或重组CHO细胞中富集的NGAL同工型的方法，包括通过二维电泳和Western印迹分析包含NGAL同工型的富集的组合物。

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公开/公告号US8394606B2

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公开/公告日2013-03-12

原文格式PDF
申请/专利权人 SALMAN ALI;RYAN M. BONN;FRANK C. GRENIER;TRACEY D. RAE;KEVIN R. RUPPRECHT;HINA N. SYED;BAILIN TU;JOAN D. TYNER;RYAN F. WORKMAN;
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申请/专利号US20090612061
发明设计人 SALMAN ALI;RYAN M. BONN;FRANK C. GRENIER;TRACEY D. RAE;KEVIN R. RUPPRECHT;HINA N. SYED;BAILIN TU;JOAN D. TYNER;RYAN F. WORKMAN;
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申请日2009-11-04
分类号C07K1/14;
国家 US
入库时间 2022-08-21 16:47:04

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