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DEVELOPMENT OF LOW COST LIQUID FORMULATION OF NATIVE STRAINS OF BACILLUS THURINGIENSIS AGAINST HELICOVERPA ARMIGERA
DEVELOPMENT OF LOW COST LIQUID FORMULATION OF NATIVE STRAINS OF BACILLUS THURINGIENSIS AGAINST HELICOVERPA ARMIGERA
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机译：苏云金芽孢杆菌天然菌株对付棉铃虫低成本菌株的研制
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摘要
A low cost liquid formulation of native strains of Bacillus thuringiensis against Helicoverpa armigera comprising step of growing, the Bacillus thuringiensis strains on media containing 2-14 ml /litre potato extract as carbon source, 2-14 gm/litre cotton seed meal as nitrogen source, 0.5 g/1 - 2.5 g/1, preferably 2.0 gm peptone, 0.5 g/1 - 2.5 g/1 preferably 1.5 g Dextrose, 0.5 g/1 - 2.5 g/ 1 preferably 2.0 gm yeast extract, 0.03 gm MgSO4, 7H2O, ,O2g FeSO4, 7H2O, .02g ZnSO4, 7H2O, 5.0g Nacl, 1ml Tween 60 and distilled water to make volume to 1 litre for 48 hrs at 30-40°C preferably at 30°C harvested cells are poured into the bottle containing sterile mustard oil, preferably -1012 cells/100 ml of liquid.
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机译：一种苏云金芽孢杆菌天然菌株针对棉铃虫的低成本液体制剂，包括在包含2-14 ml / L马铃薯提取物作为碳源，2-14 gm / L棉籽粕作为氮源的培养基上生长苏云金芽孢杆菌菌株的步骤。 ，0.5 g / 1-2.5 g / 1，优选2.0 gm蛋白ept，0.5 g / 1-2.5 g / 1优选1.5 g葡萄糖，0.5 g / 1-2.5 g / 1优选2.0 gm酵母提取物，0.03 gm MgSO4、7H2O ，，O2g FeSO4、7H2O，.02g ZnSO4、7H2O，5.0g Nacl，1ml Tween 60和蒸馏水在30-40°C，优选30°C的条件下放置48小时至1升，将收获的细胞倒入瓶中含有无菌芥末油，最好是-1012个细胞/ 100毫升液体。
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