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DEVELOPMENT OF LOW COST LIQUID FORMULATION OF NATIVE STRAINS OF BACILLUS THURINGIENSIS AGAINST HELICOVERPA ARMIGERA
DEVELOPMENT OF LOW COST LIQUID FORMULATION OF NATIVE STRAINS OF BACILLUS THURINGIENSIS AGAINST HELICOVERPA ARMIGERA
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机译：苏云金芽孢杆菌天然菌株对付棉铃虫低成本菌株的研制
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摘要
This invention relates to a low cost liquid formulation of native strains of Bacillus thuringiensis BtIII and Se against Helicoverpa armigera, the stabilizing agent used being sterile mustard oil preferably 100 ml and non food grade starch @ of 0.01% as a spreading material before spraying wherein the Bacillus thuringiensis strains are grown on production media containing 2-14 ml/litre potato extract as carbon source, 2-14 gm/litre cotton seed meal as nitrogen source, 0.5 g/1 -2.5 g/1, preferably 2.0 gm peptone, 0.5 g/1 - 2.5 g/1 preferably 1.5 g Dextrose, 0.5 g/1 - 2.5 g/1 preferably 2.0 gm yeast extract, 0.03 gm MgSO4, 7H2O, .02g FeSO4, 7H2O, .02g ZnSO4, 7H2O, 5.0g Nacl, 1ml Tween 60 and distilled water to make volume to 1 litre for 48 hrs at 30-40°C preferably at 30°C and harvested cells are poured into the bottle containing mustard oil, so that each bottle has got ~1012 cells/100 ml of liquid.
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机译：本发明涉及低成本的苏云金芽孢杆菌BtIII和Se的天然菌株对棉铃虫的液体制剂，所用的稳定剂是无菌芥末油，优选100ml，非食品级淀粉0.01％作为铺展材料，在喷雾之前，其中苏云金芽孢杆菌菌株在含有2-14 ml / L马铃薯提取物作为碳源，2-14 gm / L棉籽粉作为氮源，0.5 g / 1 -2.5 g / 1，优选2.0 gm蛋白ept，0.5的生产培养基上生长g / 1-2.5 g / 1最好是1.5 g葡萄糖，0.5 g / 1-2.5 g / 1最好是2.0 gm酵母提取物，0.03 gm MgSO4、7H2O，.02g FeSO4、7H2O，.02g ZnSO4、7H2O，5.0g Nacl，将1ml吐温60和蒸馏水在30-40°C（最好在30°C）下容量升至1升，持续48小时，然后将收集到的细胞倒入装有芥末油的瓶子中，这样每个瓶子就有〜1012个细胞/ 100毫升液体
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