首页> 外国专利> Expression vector comprising promoter, interfaces to insert use-specific gene sequence and genetic information expressing enzyme, useful to express peptide-inhibited small interfering RNA cleaving enzyme, preferably protease or peptidase

Expression vector comprising promoter, interfaces to insert use-specific gene sequence and genetic information expressing enzyme, useful to express peptide-inhibited small interfering RNA cleaving enzyme, preferably protease or peptidase

机译：包含启动子，插入用途特定基因序列和遗传信息表达酶的接口的表达载体，可用于表达肽抑制的小干扰RNA裂解酶，优选蛋白酶或肽酶

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摘要

Expression vector (1) comprising at least a promoter (2), interfaces for inserting use-specific gene (3) sequences, and a genetic information (4) expressing a peptide-inhibited small interfering RNA (siRNA) cleaving enzyme to activate peptide-inhibited siRNA, is claimed. An independent claim is included for a method of using the expression vector comprising bringing the expression vector into cells grown in a culture vessel, bringing peptide-inhibited siRNA into the cells and activating the peptide-inhibited siRNA in the cells by the expression of the enzymes that are encoded and expressed on the expression vector.

机译：表达载体（1）至少包含一个启动子（2），用于插入特定用途基因（3）序列的接口以及一个表达肽抑制小干扰RNA（siRNA）裂解酶以激活肽-的遗传信息（4）要求抑制siRNA。包括使用表达载体的方法的独立权利要求，包括将表达载体引入在培养容器中生长的细胞中，将肽抑制的siRNA引入细胞中，并通过酶的表达激活细胞中的肽抑制的siRNA。它们在表达载体上编码并表达。

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公开/公告号DE102010009445A1

专利类型
公开/公告日2011-08-25

原文格式PDF
申请/专利权人 UNIVERSITAETSKLINIKUM JENA;
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申请/专利号DE20101009445
发明设计人 POEHLMANN TOBIAS DR.;
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申请日2010-02-25
分类号C12N15/79;
国家 DE
入库时间 2022-08-21 17:47:21

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