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ANALYTICAL PLATFORM AND IDENTIFICATION METHOD WITH ANALYTES, WHICH ARE TO BE IDENTIFIED IN A SAMPLE OPTIONALLY AFTER FRACTIONATION AND WHICH SERVE AS IMMOBILIZED SPECIFIC BINDING PARTNERS
ANALYTICAL PLATFORM AND IDENTIFICATION METHOD WITH ANALYTES, WHICH ARE TO BE IDENTIFIED IN A SAMPLE OPTIONALLY AFTER FRACTIONATION AND WHICH SERVE AS IMMOBILIZED SPECIFIC BINDING PARTNERS
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机译：分馏后需要在样品中鉴定并用作固定特定结合伙伴的分析平台和鉴定方法
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摘要
The invention relates to an analytical platform and to a method carried out therewith for examining a multitude of samples for the presence of compounds, which are denoted as analytes, contained in the samples, and are biologically relevant as participants in specific binding reactions. The invention is characterized in that said samples or fractions of these samples along with the analytes, which are to be identified and contained therein and which serve as a first multitude of specific binding partners, are placed in discrete measuring areas in at least one one-dimensional or two-dimensional array on an evanescent field sensor platform, which serves as a solid support, directly or after additional dilutions of the samples or of the fractions. Different samples or fractions or different dilutions of samples or fractions are arranged in different discrete measuring areas. One or more identifying substances, which serve as a second multitude of specific binding partners and which are provided for specifically identifying one or more analytes contained in the samples that are from said first multitude of specific binding partners, are, in a single or a number of steps of a specific binding reaction, brought into contact with the samples or their fractions or their dilutions placed in said discrete measuring areas. Changes in optoelectronic signals due to the binding of identifying substances to analytes contained in discrete measuring areas are measured in a locally resolved manner in the evanescent field of the evanescent field sensor platform, and the presence of the analytes to be specifically identified in the respective measuring areas is determined qualitatively and/or quantitatively on the basis of the relative magnitude of the changes in said optoelectronic signals from the respective measuring areas.
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