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IDENTIFICATION OF THE POWDERED SILKWORM STRAINS BY THE DIRECT SEQUENCE ANALYSIS OF INTRONIC REGIONS
IDENTIFICATION OF THE POWDERED SILKWORM STRAINS BY THE DIRECT SEQUENCE ANALYSIS OF INTRONIC REGIONS
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机译:通过内向区的直接序列分析鉴定粉状蚕丝菌株
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摘要
Provided is a method for discriminating domestic silkworm powder from imported silkworm powder through direct base sequencing analysis. Nine introns are used as silkworm discrimination markers. The nine introns indicate A4Intron1, A4Intron2, PTTHIntron3, LCP30Intron3, LCP30Intron4, SPIIntron3, VDPIntron4, FLCIntron3 and XDHIntron3. A method for discriminating domestic silkworm powder using the direct base sequencing analysis comprises the following steps of: extracting a genome DNA from the silkworm powder; offering information about DNA amplification existence or nonexistence in a specific intron region through PCR(polymerase chain reaction); offering information about discriminable intron regions based on species and primer in the regions through the direct base sequencing analysis; and comparing the species in the intron regions using the silkworm discrimination marker.
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