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IDENTIFICATION OF THE POWDERED SILKWORM STRAINS BY THE DIRECT SEQUENCE ANALYSIS OF INTRONIC REGIONS

机译:通过内向区的直接序列分析鉴定粉状蚕丝菌株

摘要

Provided is a method for discriminating domestic silkworm powder from imported silkworm powder through direct base sequencing analysis. Nine introns are used as silkworm discrimination markers. The nine introns indicate A4Intron1, A4Intron2, PTTHIntron3, LCP30Intron3, LCP30Intron4, SPIIntron3, VDPIntron4, FLCIntron3 and XDHIntron3. A method for discriminating domestic silkworm powder using the direct base sequencing analysis comprises the following steps of: extracting a genome DNA from the silkworm powder; offering information about DNA amplification existence or nonexistence in a specific intron region through PCR(polymerase chain reaction); offering information about discriminable intron regions based on species and primer in the regions through the direct base sequencing analysis; and comparing the species in the intron regions using the silkworm discrimination marker.
机译:提供了一种通过直接碱基测序分析来区分进口蚕粉和国产蚕粉的方法。九个内含子用作家蚕识别标记。九个内含子分别表示A4Intron1,A4Intron2,PTTHIntron3,LCP30Intron3,LCP30Intron4,SPIIntron3,VDPIntron4,FLCIntron3和XDHIntron3。一种利用直接碱基序列分析法鉴别家蚕粉的方法,包括以下步骤:从家蚕粉中提取基因组DNA。通过PCR(聚合酶链反应)提供有关特定内含子区域DNA扩增存在或不存在的信息;通过直接碱基测序分析提供基于物种和区域内引物的可区分内含子区域的信息;并使用家蚕区分标记比较内含子区域中的物种。

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