METHODS OF REDUCING REPEAT-INDUCED SILENCING OF TRANSGENE EXPRESSION AND IMPROVED FLUORESCENT BIOSENSORS

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  机译： 减少转基因表达的重复诱导沉默的方法和改进的荧光生物传感器

• 4. METHODS OF REDUCING REPEAT-INDUCED SILENCING OF TRANSGENE EXPRESSION AND IMPROVED FLUORESCENT BIOSENSORS [P] . 欧洲知识产权局专利： EP1943263A2 . 2008-07-16

  机译： 减少转基因表达的重复诱导沉默和改良荧光生物传感器的方法

• 5. METHODS OF REDUCING REPEAT-INDUCED SILENCING OF TRANSGENE EXPRESSION AND IMPROVED FLUORESCENT BIOSENSORS [P] . 世界知识产权组织专利： WO2007046787A2 . 2007-04-26

  机译： 减少转基因表达的重复诱导沉默和改良荧光生物传感器的方法

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• 1. Analytical Expressions for Steady-State Concentrations of Substrate and Product in an Amperometric Biosensor with the Substrate Inhibition—The Adomian Decomposition Method [J] . Anandan Anitha ,Shunmugham Loghambal ,Lakshmanan Rajendran . 美国分析化学（英文） . 2012,第8期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Flash Lamp Annealing Method for Improving Adhesion Strength on the Dielectric Material and Reducing Substrate Warpage [J] . Jong-Young Park ,Byeong-Jae Choi ,Young-Jae Kim . 材料科学与工程：中英文B版 . 2019,第003期
• 4. 反向重复转基因不同位置侧翼序列诱导的基因沉默差异比较 [C] . 孔令产 ,景茂峰 ,公娇芬 . 中国植物病理学会2010年学术年会 . 2010
• 5. 转基因五指山小型猪在诱导Ⅰ型糖尿病模型及其后代的红色荧光蛋白基因表达上的应用 [A] . 洪禹 . 2014