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RECOMBINANT VP2 PROTEIN COMPRISING A CANINE PARVOVIRUS NEUTRALIZATION ANTIGEN EPITOPE AND VACCINE COMPOSITION COMPRISING THE SAME TO EFFECTIVELY PREVENT AND TREAT INFECTION OF CANINE PARVOVIRUS
RECOMBINANT VP2 PROTEIN COMPRISING A CANINE PARVOVIRUS NEUTRALIZATION ANTIGEN EPITOPE AND VACCINE COMPOSITION COMPRISING THE SAME TO EFFECTIVELY PREVENT AND TREAT INFECTION OF CANINE PARVOVIRUS
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机译：包含犬细小病毒中和抗原表位的重组VP2蛋白和有效组成和预防犬细小病毒感染的疫苗组合物
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摘要
PURPOSE: A recombinant VP2 protein comprising a canine Parvovirus neutralization antigen epitope and a vaccine composition comprising the same protein are provided, thereby effectively preventing and treating infection of canine Parvovirus. CONSTITUTION: The recombinant VP2 protein comprising a canine Parvovirus neutralization antigen epitope comprises the amino acid sequence selected from SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25 and SEQ ID NO:29. An expression vector pGEM-4T contains a gene encoding the recombinant VP2 protein. A method for producing the recombinant VP2 protein comprises the steps of: (a) constructing an expression vector containing the gene encoding the recombinant VP2 protein operatively linked to an expression control sequence; (b) transforming a host cell with the recombinant expression vector; and (c) culturing the transformed cell in a medium and recovering the substantially pure recombinant VP2 protein.
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机译：目的：提供一种包含犬细小病毒中和抗原表位的重组VP2蛋白和包含该蛋白的疫苗组合物，从而有效地预防和治疗犬细小病毒的感染。组成：包含犬细小病毒中和抗原表位的重组VP2蛋白，其氨基酸序列选自SEQ ID NO：17，SEQ ID NO：19，SEQ ID NO：21，SEQ ID NO：23，SEQ ID NO：25和SEQ ID NO：29。表达载体pGEM-4T包含编码重组VP2蛋白的基因。生产重组VP2蛋白的方法包括以下步骤：（a）构建表达载体，该表达载体包含与表达控制序列可操作连接的编码重组VP2蛋白的基因; （b）用重组表达载体转化宿主细胞; （c）在培养基中培养转化的细胞并回收基本上纯的重组VP2蛋白。
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