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NUCLEIC ACID MOLECULES ENCODING ISOPENTENYL MONOPHOSPHATE KINASE, AND METHODS OF USE
NUCLEIC ACID MOLECULES ENCODING ISOPENTENYL MONOPHOSPHATE KINASE, AND METHODS OF USE
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机译：编码异戊烯酸单磷酸激酶的核酸分子及其使用方法
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摘要
A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Menthax piperita) has been isolatedand sequenced, and the corresponding amino acid sequence has been determined.Accordingly, an isolated DNA sequence (SEQ IDNO:1) is provided which codes for the expression of isopentenyl monophosphatekinase (SEQ ID NO:2), from peppermint (Mentha xpiperita). In other aspects, replicable recombinant cloning vehicles areprovided which code for isopentenyl monophosphate kinase,or for a base sequence sufficiently complementary to at least a portion ofisopentenyl monophosphate kinase DNA or RNA to enablehybridization therewith. In yet other aspects, modified host cells areprovided that have been transformed, transfected, infected and/orinjected with a recombinant cloning vehicle and/or DNA sequence encodingisopentenyl monophosphate kinase. Thus, systems andmethods are provided for the recombinant expression of the aforementionedrecombinant isopentenyl monophosphate kinase thatmay be used to facilitate its production, isolation and purification insignificant amounts. Recombinant isopentenyl monophosphatekinase may be used to obtain expression or enhanced expression of isopentenylmonophosphate kinase in plants in order to enhancethe production of isopentenyl monophosphate kinase, or isoprenoids derivedtherefrom, or may be otherwise employed for the regulationor expression of isopentenyl monophosphate kinase, or the production of itsproducts.
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机译：薄荷（Mentha）中编码异戊烯基单磷酸激酶（IPK）的cDNAx piperita）已被隔离并测序，并确定了相应的氨基酸序列。因此，分离的DNA序列（SEQ ID提供NO：1），用于编码异戊烯基单磷酸的表达薄荷（Mentha x）的激酶（SEQ ID NO：2）piperita）。在其他方面，可复制的重组克隆载体是提供了异戊烯基单磷酸激酶的编码，或对于与至少一部分异戊烯基单磷酸激酶DNA或RNA使能与之杂交。在其他方面，修饰的宿主细胞是只要已经被转化，转染，感染和/或注射重组克隆载体和/或编码异戊烯基单磷酸激酶。因此，系统和提供了用于前述表达的重组表达的方法重组异戊烯基单磷酸激酶可用于促进其生产，分离和纯化大量。重组异戊烯基单磷酸酯激酶可用于获得异戊烯基的表达或增强表达为了增强植物中的单磷酸激酶异戊烯基单磷酸激酶或类异戊二烯衍生物的产生以此为准，或可以其他方式用于监管或异戊烯基单磷酸激酶的表达或其产生产品。
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