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INDUCTION OF MUCOSAL IMMUNE RESPONSES BY VITAMIN D3 AND ANTIGEN-ENCODING DNA COMPLEXE TO CATIONIC LIPID
INDUCTION OF MUCOSAL IMMUNE RESPONSES BY VITAMIN D3 AND ANTIGEN-ENCODING DNA COMPLEXE TO CATIONIC LIPID
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机译：维生素D3诱导粘膜免疫反应和抗原标记的DNA复合物对阳离子脂质的诱导
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摘要
The invention provides a method of inducing a mucosal immune response in a subject, comprising administering to the subject an amount of antigen-encoding DNA effective to induce a mucosal immune response complexed to a transfection-facilitating cationic lipid and an amount of vitamin D3. In the method of inducing a mucosal immune response, the antigen-encoding DNA can encode an antigen that is expressed on the surface of transfected cells and mimic critical elements of infection. DNA encoding the envelope glycoproteins of viral pathogens is used in the present method. Cationic lipids are bifunctional molecules consisting of one or more hydrophobic chains covalently linked to a cationic grouping in which there is coordination of positively charged groups with a negatively charged phosphate oxygen of the DNA chain forming an ionic charge complex. Two preferred examples of cationic lipids are DOGS (dioctadecylamidoglycylspermidine) and TEDBI (N,N,N',N'-tetramethyl N,N'-bis(2-hydroxyethyl)-2,3-dioleoyloxy-1,4-butanediammonium iodide). The invention also provides a composition, comprising an amount of DNA encoding an envelope antigen or envelope-associated antigen of a pathogen complexed to a cationic lipid. More specifically, the invention provides a composition, comprising an amount of DNA encoding an envelope antigen of HIV complexed to a cationic lipid.
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