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Method of assessing the triticale genotypes resistance to root fusariose

机译:评估小黑麦基因型对根镰刀菌抗性的方法

摘要

The invention refers to agriculture and may be used for assessment of triticale genotypes resistance to the root fusariose.The proposed method includes determination of explant callusing, cultivated on the nutrient medium N6, adding culture filtrate of the fungi Fusarium oxysporum or F. graminearum in the amount of 20…30% of the total volume of the medium. In the capacity of explant there are used the anthers of the triticale genotypes. The genotypes, to which the anther callusing compared with the control constitutes 32…66%, are being assessed as resistant.The result of the invention consists in increasing the accuracy and in the assessment acceleration of the triticale genotypes resistance to root fusariose.
机译:本发明涉及农业,可用于评估黑小麦基因型对根福萨糖的抗性。提出的方法包括测定外植体愈伤组织,在营养培养基N6上栽培,在真菌中添加尖孢镰刀菌或禾谷镰孢的培养滤液。占培养基总体积的20…30%。在外植体方面,使用了黑小麦基因型的花药。与对照相比,花药call愈的构成为32…66%的基因型被评估为抗性。本发明的结果在于提高了黑小麦基因型对根福寿糖抗性的准确性和评估加速度。

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